基质金属蛋白酶2,3,13基因表达水平与牛肉质性状的相关性分析

系水力、剪切力和蒸煮损失是肉质性状的重要指标,肌纤维细胞外基质的成分和结构对肉质性状有重要的影响,基质金属蛋白酶(MMPs)是体内降解细胞外基质的蛋白酶。本研究采用qRT-PCR技术分析了南阳牛肌肉发育过程中MMP2,3,13基因的表达变化规律,发现MMP2和MMP3基因在18月龄时表达量显著降低(P0.05),MMP13基因表达水平在整个发育期间无显著差异。采集了24头2岁左右的南阳牛与夏洛莱F1代杂交牛背最长肌组织,分别测定了系水力、剪切力、蒸煮损失和肌内脂肪含量,发现MMP2基因的表达量与剪切力间呈显著负相关(r=-0.542 0,P0.01);MMP13基因的表达量与系水力间呈显著负相关(r=-0.489 1,P0.05)。结合已有研究,可初步推断MMPs基因可能通过调控胶原蛋白等细胞外基质成分的重构而影响肉质。

The Expression of MMP2,3,13 Genes in Skeletal Muscle Tissues and Its Relationship with Meat Quality in Cattle

Water holding capacity, shearing force and cooking loss were the main meat quality traits, which affected greatly by the component and construction of extracellular matrix around muscle fiber.Matrix metalloproteinases (MMPs) regulated the remodelling of extracellular matrix in vivo.The expression of MMP2,3,13 were analyzed by qRT-PCR during the development of longissimus dorsi muscle and results showed that MMP2 and MMP3 had significant lower expression levels at 18 month old (P0.05), while the MMP13 had no significant change. Twenty-four longissimus dorsi muscle samples of F1 hybrid progeny of Nanyang cattle Charolais cattle were collected to test the water holding capacity, shearing force, cooking loss and intramuscular fat content at two years old. The expression of MMP2 had significant negative correlation relationship with shearing force (r=-0.542 0,P0.01), and the expression of MMP13 had significant negative correlation relationship with water holding capacity (r=-0.489 1,P0.05). This study indicated the MMPs could affect the meat quality by regulation the remodelling of extracellular matrix.