低温和超低温保存花粉对番茄坐果率和种子量的影响

为了研究不同处理下长期保存花粉对番茄坐果率和种子量的影响,本文以云南农业大学园林园艺学院选育的Ryau96172I和Ryau9327D两个番茄品系为试材,采用低温(4℃,处理I)及超低温(-196℃,处理II)条件保存花粉,以常温作为对照(CK),对不同处理的花粉人工授粉(60朵花)后的坐果率、种子总重量、平均单果种子重量、种子总数及单果种子平均数进行比较分析.结果显示:与对照相比,处理I和处理II人工授粉后对番茄的坐果率无显著差异;处理I人工授粉后番茄的种子总重量、平均单果种子重量、种子总数及单果种子平均数无明显差异,但处理II人工授粉后其相应指标显著偏低(P0.05).结果表明: 超低温处理下保存花粉对番茄的种子量有较大影响.

Effects of Low Temperature and Cryopreservation on Fruit Set Percentage and Seed Yield of Tomato

In order to study the effects of different treatments on the fruit set percentage and seed yield of tomato, based on the breeding of Ryau96172I and Ryau9327D tomato strains as test materials in Yunnan Agricultural University College of Landscape and Horticulture, low temperature (4℃, treatment I ) and cryopreservation (-196℃, treatment II) conditions were adopted to preserve pollen, meanwhile, contrasting to the normal atmospheric temperature (CK), the fruit set percentage, fruit seed weight, average single fruit seed weight, the amount of seeds and average seeds per fruit were compared and analyzed after supplemental pollination. The results showed that compared with the control,treatment I and treatment II had no significant effect on fruit set percentage.Treatment I had no significant effect with contrast on fruit seed weight, average single fruit seed weight, the amount of seeds and average seeds per fruit; but treatment II showed the results significantly lower compared with the control.The result above showed that the conserving the pollen had an important effect on seed yield of tomato.