云南省十字花科蔬菜根肿病的实时荧光定量PCR检测

根肿病是十字花科蔬菜严重的土传病害之一。为快速和准确的检测根肿病菌在土壤中的含量,本研究利用实时荧光定量PCR技术,结合传统的生物学测定方法对云南省十字花科蔬菜基地22份土壤样品进行了检测。结果表明:土壤孢子数量和病情指数呈线性相关,随着土壤中根肿病孢子数量增加,生物学测定的病情指数也随之增加;所检测的土壤中孢子密度最高为110sup8.96 g-1,病情指数为95.56%,最低为1103.12 g-1,相应的病情指数为3.33%。该方法可以快速检测和定量土壤中病原菌,为种植十字花科蔬菜土壤的检测预报提供了有效的技术手段。

Detection of Plasmodiophora brassicae with Real-time Quantitative PCR in Yunnan Province

Clubroot of crucifereais vegetables is one of the serious soil-borne diseases. The quickly and quantify detection of Plasmodiophora brassicae of 22 cruciferous vegetables soil samples in Yunnan were tested by using real-time quantitative PCR technology and traditional method. The results showed that the highest concentration of clubroot spores was 1 108.96 g-1 while the disease index of 95.56%;the lowest concentration of clubroot spores was 1 103.12 g-1 while the disease index of 3.33%. The disease index was linearly correlated with clubroot spores number. The disease index and the number clubroot spores were increased or decreased at the same time. This method can quickly and quantify detect the P.brassicae in soil and provide an effective techniques for cruciferous vegetables soil detection.