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| 大白菜PAT1基因的克隆及其表达载体的构建 | |
| 引用本文： | 徐凤,宋梅芳,王保,高玲,孙阳,高建伟. 大白菜PAT1基因的克隆及其表达载体的构建[J]. 山东农业科学, 2009, 0(2): 1-4 |
| 作者姓名： | 徐凤 宋梅芳 王保 高玲 孙阳 高建伟 |
| 作者单位： | 1. 青岛农业大学生命科学学院,山东,青岛,266109 2. 山东省设施蔬菜生物学重点实验室/山东省农业科学院蔬菜研究所,山东,济南,250100 |
| 基金项目： | 国家科技基本条件平台建设项目，山东省农业科学院高技术自主创新基金 |
| 摘 要： | 根据GenBank中大白菜（Brassica campestris L ssp．pekinensis）基因组测序结果，分析了大白菜PAT1（phytochrome A sjgnal transduction）基因的内含子和外显子。通过拼接外显子，获得了大白菜PAT1基因的cDNA编码序列。根据编码区序列，设计并合成了一对引物；利用这对引物，采用RT—PCR技术扩增出大白菜福山包头PAT1基因（CcPAT1）的全长cDNA序列。CcPATl编码区共1500bp，与拟南芥PAT1相似性为85．03％。同时构建了含有35S启动子的pCAMBIA-2300-CcPAT1过表达载体，并利用以PAT1 5’端特异区域构建了pFGC-1008-PAT1RNAi（RNA interference）载体，以备将来研究大白菜CcPAT1基因的功能。 |
| 关 键 词： | 大白菜 PAT1基因 cDNA克隆 表达载体 RNAi |
Clone of PAT1 Gene in Chinese Cabbage and Construction of Its Expression Vectors |
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| XU Feng,SONG Mei-fang,WANG Bao,GAO Ling,SUN Yang,GAO Jian-wei. Clone of PAT1 Gene in Chinese Cabbage and Construction of Its Expression Vectors[J]. Shandong Agricultural Sciences, 2009, 0(2): 1-4 | |
| Authors: | XU Feng SONG Mei-fang WANG Bao GAO Ling SUN Yang GAO Jian-wei |
| Affiliation: | 1.College of Life Science;Qingdao Agricultural University;Qingdao 266109;China;2.Shandong Key Laboratory of Greenhouse Vegetable Biology/Institute of Vegetables;Shandong Academy of Agricultural Sciences;Jinan 250100;China |
| Abstract: | The intron and exon of PAT1(phytochrome A signal transduction) gene in Chinese cabbage(Brassica campestris L.ssp. pekinensis) were analyzed based on the genomic DNA sequence in GenBank.By splicing the exons,the cDNA sequence of PAT1 gene was obtained.Then a pair of primers was designed for RT-PCR.The results showed that the full-length CcPAT1 cDNA of Chinese cabbage variety Fushanbaotou was isolated,which was 1 500 bp and 85.03% homologous to the Arabidopsis thaliana PAT1 gene. To study the functions of CcP... |
| Keywords: | RNAi |
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