| 荔枝快速碱化因子基因的克隆与表达分析 |
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| 引用本文： | 王家保,贾彩红,杨小亮,金志强,徐碧玉.荔枝快速碱化因子基因的克隆与表达分析[J].热带作物学报,2009,30(12):1798-1802. |
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| 作者姓名： | 王家保 贾彩红 杨小亮 金志强 徐碧玉 |
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| 作者单位： | 1. 中国热带农业科学院环境与植物保护研究所,海南儋州,571737;中国热带农业科学院热带生物技术研究所,海南海口,571101
2. 中国热带农业科学院热带生物技术研究所,海南海口,571101 |
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| 基金项目： | 国家自然科学基金，中央级科研院所基本业务费项目，国家荔枝产业技术体系专项经费资助 |
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| 摘 要： | 从已构建的荔枝果皮cDNA文库中挑取克隆测序,获得一条荔枝快速碱化因子(LcRALF)基因(GenBank登录号:EU024484).该基因的cDNA全长为666 bp,含1个381 bp的开放读码结构,编码126个氨基酸.生物信息学分析表明,该基因的氨基酸序列与拟南芥、烟草等物种的RALF基因一致性较高,具有RALF基因氨基酸序列的典型特征.RT-PCR分析表明,该基因在荔枝果皮中特异表达,且在新采收的荔枝果皮中表达量最高.随着果皮的衰老该基因表达量逐渐下降.
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| 关 键 词： | 荔枝 果皮 快速碱化因子 基因克隆 基因表达 |
Cloning and Expression Analysis of LcRALF Gene from Litchi |
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| Wang Jiabao,Jia Caihong,Yang Xiaoliang,Jin Zhiqiang and Xu Biyu.Cloning and Expression Analysis of LcRALF Gene from Litchi[J].Chinese Journal of Tropical Crops,2009,30(12):1798-1802. |
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| Authors: | Wang Jiabao Jia Caihong Yang Xiaoliang Jin Zhiqiang and Xu Biyu |
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| Abstract: | A novel rapid alkalinization factor gene(LcRALF) of litchi was isolated from cDNA library of litchi pericarp(GenBank accession No.:EU024484). The LcRALF cDNA was 666 bp in full length with an open reading frame of 381 bp, which encoded a 126 aa polypetide. Amino acid sequence of LcRALF had high identities with RALF genes from Arabidopsis thaliana, Nicotiana tabacum and other plant species and had the same structure characteristics as other RALF genes. RT-PCR analysis showed that LcRALF was expressed specially in litchi pericarp and had the highest expression level in the pericarp of freshly-har-vested litchi fruit. With the senescence of pericarp, expression level of LcRALF decreased gradually. |
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| Keywords: | litchi RALF gene cloning gene expression |
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