"循化红"线辣椒SRAP-PCR反应体系的优化与建立

目的]建立适合于“循化红”线辣椒基因组的SRAP-PCR优化扩增反应体系。方法]采用L16（4^5）正交试验设计，对“循化红”线辣椒SRAP反应体系中的Mg^2＋浓度、Taq聚合酶、dNTPs浓度、引物浓度、模板DNA浓度进行5因素4水平正交优化。结果]“循化红”线辣椒的最佳SRAP—PCR反应体系为：Mg^2＋浓度为2．0mmol／L，dNTPs为0．5mmol／L，Taq酶0．5U，引物浓度为0．4μmol／L，模板DNA浓度为1．0ng/μl，总体积20μl。结论]该体系的建立为SARP标记技术应用于“循化红”线辣椒种质资源的收集与利用，品种的鉴定、标记辅助育种等方面奠定了良好的试验基础。

Optimization and Establishment for SRAP-PCR System of Line Pepper Xunhuahong

Objective]The research aimed to establish the suitable SRAP-PCR system of line pepper "Xunhuahong".Method]Orthogonal design was used to optimize SRAP-PCR amplification system of line pepper on four levels of five factors (Taq DNA polymerase, Mg~(2+), DNA template, dNTPs, and primer respectively).Result]A most suitable SRAP-PCR system for line pepper was established.And the total reaction system was 20 μl contained 0.5 U DNA polymerase, 2.0 mmol/L Mg~(2+), 1.0 ng/μl DNA template, 0.5 mmol/L dNTPs, and 0.4 μmol/L primer. Conclusion]This optimized system for SRAP marker could provide good basis for further research on germplasm resources collection and utilization, variety identification and marker-assisted selection in line pepper.