日本凹顶藻的活性物质对姬松茸病原菌的抑制作用研究

通过对日本凹顶藻以不同溶剂的粗提、乙醇提取物的极性分离、不同极性的薄层层析和多糖的提取，进行抗菌试验的研究，发现95%乙醇和V甲醇：甲苯为3∶1的提取的活性物质具有较强的抗姬松茸的病原细菌活性，乙酸乙酯相对病原细菌的抑菌强于乙醇的粗提物，而水相基本无抑菌效果，乙酸乙酯相的两个带（Rf0.57、Rf0.63）浓度为0.1g/ml具有较强抗姬松茸病原细菌的活性，对假单孢杆菌抑菌圈达到19.3mm，水相层析带的抑菌活性较弱，多糖的粗提物配成0.25g/g的水溶液对姬松茸病原真菌有较好的抑菌效果对黑曲霉、桔青霉和绿色木霉的抑菌圈分别达到10.8 mm、7.9 mm、5.3 mm。

Inhibitory Effect of Active Substance of Laurencia Nipponica Yamada on Pathogenic Bacillus of Agaricus blazei Murril

The antimicrobial test and researched was done on wide distillation of different solvents on Terpenoid, polarity separation of ethanol distillable substance, thin layer chromatography of different polarities and distillation of amylase. The results showed that the active substance distilled by 95% ethanol and V methanol: toluene=3:1 had stronger antimicrobial activity on Agaricus blazei Murrill. The antimicrobial activity of ethyl acetate was stronger than wide distilling substance with ethanol, but the water part has not antimicrobial effect. Two straps of ethyl acetate phase（Rf0.57、Rf0.63）(0.1g/ml)had stronger antimicrobial activity on Agaricus blazei Murrill, the antimicrobial zone on Pseudomonas was 19.3mm. The bacteriostatic activity of chromatography strap of water phase was rather weaker, the water solution (0.5g/g) of amylase had better effect on pathogenic fungus of Agaricus blazei Murrill, its antimicrobial zone on Aspergillus niger, Citrinin, Trichoderma viride were 10.8 mm, 7.9 mm, 5.3 mm.