| 异源六倍体小黑麦愈伤组织的诱导与再生分化植株的研究 |
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| 引用本文： | 刘世强,杨从荣,等.异源六倍体小黑麦愈伤组织的诱导与再生分化植株的研究[J].沈阳农业大学学报,1992,23(1):1-6. |
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| 作者姓名： | 刘世强 杨从荣 |
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| 作者单位： | 沈阳农业大学农学系 |
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| 摘 要： | 以异源六倍体小黑麦幼穗作外植体，愈伤组织诱导培养基内2，4－D浓度调节在2．0－2．5mg/l之间，其诱导效果最佳，接种后6天后的诱导率达70％，9天后达95％，在继代培养其中2，4－D浓度调到3．5－4．0mg/l之间，继代的愈伤组织发育良好，质量高，在分化培养基中6－BA，KT，IAA的组配比例为1：0．99：0．3时，能提高分化率，一般可达80％以上。
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| 关 键 词： | 小麦 六倍体 愈伤组织 诱导 再生株 |
Induction and Differentiation of Embryogenetic Callus of AIIohexoploid Triticale |
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| Liu Shiqiang Yang Congrong Wang Shuling.Induction and Differentiation of Embryogenetic Callus of AIIohexoploid Triticale[J].Journal of Shenyang Agricultural University,1992,23(1):1-6. |
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| Authors: | Liu Shiqiang Yang Congrong Wang Shuling |
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| Abstract: | The young panicle of allohexoploid triticale was used as explant.The best medium for callus induction was 2,4-D ranged from 2. 0 to2. 5mg/1. Induction frequency was 70% after 6 days,and 95% after 9 days of induction.When the 2,4-D concentration was raisen from3. 5mg/1 to 4. 0mg/1 during subculture of callus,the tissues grew better.The differentiation frequency of the plant reached more than 80% during differentiation culture when the three hormones were properly combined (6-BA:KT:IAA=1:0. 9:0. 3) . |
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| Keywords: | allohexaploid triticale young Dunicle explant embryogenetic callus |
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