A simple assay system incorporating monoclonal antibodies for the detection of potato virus T |
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| Authors: | Mairi L. Vernon-Shirley Robert Burns Edna L. George Margaret E. Hoadley |
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| Affiliation: | (1) Scottish Agricultural Science Agency, Craigs Road, East Craigs, EH12 8NJ Edinburgh, Scotland UK |
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| Abstract: | Summary Two monoclonal antibodies (McAbs) which react specifically with potato virus T (PVT) were produced and tested for cross reactivity with potato viruses (A, S, X, Yo, YN, leaf roll), apple stem grooving virus (ASGV) and against healthy sap. They were found to be highly specific for PVT. These two McAbs form the basis of an enzyme-linked immunosorbent assay (ELISA) for the detection of PVT in infected plant material which is of key significance for potato quarantine. |
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| Keywords: | ELISA hybridoma closterovirus Solanum tuberosum L. |
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