| 含绿色荧光蛋白基因的鸭肠炎病毒SD-01株US2基因缺失转移载体的构建 |
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| 引用本文： | 李子剑,李云龙,陈苏,李玉峰.含绿色荧光蛋白基因的鸭肠炎病毒SD-01株US2基因缺失转移载体的构建[J].水禽世界,2007(11):14-16. |
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| 作者姓名： | 李子剑 李云龙 陈苏 李玉峰 |
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| 作者单位： | 1. 山东师范大学生命科学学院,济南,250023
2. 山东省农业科学院家禽研究所禽病研究中心 |
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| 摘 要： | 以鸭肠炎病毒(DEV)SD-01株DNA为模板,根据Gen Bank上已发表的基因序列设计一对引物,利用PCR技术扩增出US2全基因,将PCR产物连入pGM-T载体。用BamHI和EcoR V双酶切重组载体,使US2基因缺失约130bp,将含双loxP酶切位点的EGFP完整表达盒插入BamH I和EcoR V酶切位点之间,成功构建了以US2基因为重组臂的含EGFP基因的转移质粒载体pUS2-loxP-EGFP-loxP。
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| 关 键 词： | 鸭肠炎病毒 US2基因 EGFP基因 转移载体 |
| 文章编号： | 1673-1085（2007）11-0014-03 |
| 收稿时间： | 2007-10-30 |
Construction of US2 gene-deleted DEV SD-01 straintransfer vector containing EGFP |
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| LI Zi-jian,LI Yun-long,CHEN Su,LI Yu-feng.Construction of US2 gene-deleted DEV SD-01 straintransfer vector containing EGFP[J].Poultry Science,2007(11):14-16. |
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| Authors: | LI Zi-jian LI Yun-long CHEN Su LI Yu-feng |
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| Institution: | 1.College of Life Science of Shandong Normal University, Jinan 250023;2.Poultry Institute of Shandortg Academy of Agricultral Science,Jinan Shandong 250023,china |
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| Abstract: | DEV SD-01 strain genomic DNA was extracted and a pair of primers was synthesized according to the published nucleotide sequence in GenBank. The US2 gene was amplified by polymerase chain reaction (PCR) with extracted total DNA from DEV SD-01 strain as template and was cloned into vector pGM-T. The EGFP expression cassette flanked by two loxP sites was inserted between BamH I and EcoR V sites of the US2 gene to give rise to the transferring vector pUS2-loxP-EGFP-loxP. |
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| Keywords: | Duck enteritis virus US2 gene EGFP gene transferring vector |
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