甘菊下胚轴离体再生体系的建立

以甘菊下胚轴为外植体，通过器官发生途径诱导不定芽分化，建立了甘菊下胚轴高效再生体系，为甘菊遗传转化体系的建立奠定了基础。结果表明:将甘菊种子播种于MS培养基上，置于黑暗条件下培养7 d可以迅速获得大量下胚轴；下胚轴在MS+1.0 mg/L 2, 4-D+0.5 mg/L 6-BA的培养基上培养15 d后，愈伤组织形成率最高为8666%，愈伤组织呈淡黄色，大小一致；将愈伤组织转接到MS培养基中培养30 d左右即可分化不定芽，不定芽分化率达25.50%，平均每个外植体产生不定芽数目为4.25个；不定芽在添加0.1 mg/L NAA的1/2 MS培养基中培养15 d后，生根率达到100%。生根试管苗出瓶后，经过适宜培养，均可以获得健壮的开花植株。 

Establishing an in vitro regeneration system from hypocotyls of Chrysanthemum lavandulifolium.

An efficient regeneration protocol had been established for plantlet regeneration from hypocotyl-induced calli of Chrysanthemum lavandulifolium,which laid the foundation for genetic transformation of C.lavandulifolium.When long hypocotyls from the seeds that germinated in the dark for 7 days were cultured on the Murashige and Skoog’s(MS) medium containing 1.0 mg/L 2,4-dichlorophenoxyacetic acid(2,4-D) and 0.5 mg/L 6-benzyl aminopurine(6-BA) for 15 days,the calli inducing rate could reach the maximum of 86.66%.The calli were light yellow with almost uniform size.Following approximately 30 days of culture on the MS medium,adventitious buds were differentiated.The highest adventitious bud rate(25.50%) with an average of about 4.25 adventitious buds per explant was obtained from calli cultured on a MS medium without 6-BA.The rooting rate was 100% when the adventitious buds were cultured on the 1/2 MS medium supplemented with 0.1 mg/L naphthaleneacetic acid(NAA) for 15 days.After acclimatized to greenhouse conditions,normal growth and blossom C.lavandulifolium plants were obtained.