| 副猪嗜血杆菌间接ELISA抗体检测方法的建立 |
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| 引用本文： | 王艳,夏万田,柏坤桃,尹秀凤,姜平.副猪嗜血杆菌间接ELISA抗体检测方法的建立[J].畜牧与兽医,2006,38(3):5-7. |
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| 作者姓名： | 王艳 夏万田 柏坤桃 尹秀凤 姜平 |
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| 作者单位： | 1. 南京农业大学动物医学院农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210095
2. 江苏省高邮市畜牧兽医站,江苏,高邮,225600 |
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| 基金项目： | 浙江省科技攻关项目(021102529)，教育部博士点基金项目(20030307012)，教育部优秀青年教师资助计划，教育部重点项目(104101)。 |
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| 摘 要： | 采用福尔马林灭活的副猪嗜血杆菌全菌体作为包被抗原,建立了检测副猪嗜血杆菌抗体的间接ELISA方法。经试验确定副猪嗜血杆菌全菌体的包被浓度为2·24×107CFU/孔、检测血清为1∶200稀释,同时确立了间接ELISA的最佳反应条件。该方法有很高的特异性和重复性,14个发病猪场100份血清检测结果Hps抗体阳性检出率为94%,明显高于细菌分离鉴定检测结果。
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| 关 键 词： | 副猪嗜血杆菌 ELISA 抗体 |
| 文章编号： | 0529-5130（2006）03-0005-03 |
| 收稿时间： | 2005-08-29 |
| 修稿时间： | 2005年8月29日 |
Development of an indirect ELISA for detection of antibodies against Haemophilus parasuis |
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| WANG Yan,XIA Wan-tian,BO Kun-tao,YIN Xiu-feng,JIANG Ping.Development of an indirect ELISA for detection of antibodies against Haemophilus parasuis[J].Animal Husbandry & Veterinary Medicine,2006,38(3):5-7. |
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| Authors: | WANG Yan XIA Wan-tian BO Kun-tao YIN Xiu-feng JIANG Ping |
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| Abstract: | Haemophilus parasuis inactivated with 0.2% formalin was applied as antigen, and an indirect ELISA was developed by determination of the optimal reaction condition to monitor serum antibody levels in pigs. The optimal antigen dilution and serum dilution were further examined as following: The final concentration of the antigen was 2.24×10~7 CFU/well (diluted in carbonate sodium buffer) and the dilution of the serum sample was 1∶200. It was proved that the method had high specificity to Haemophilus parasuis (Hps). The positive rate of 100 clinical sera samples from 14 pig herds was 94%, higher than that of Hps isolation. |
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| Keywords: | Haemophilus parasuis ELISA antibody |
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