| Abstract: | On the basis of previous studies dealing with the variation of major
agronomic and yield characteristics of regenerated plants derived from single cell culture in
vitro of common wheat (Triticum aestivum L.Cultivar NE 7742), the grain protein content and
its fractions from regenerated plants with stable agronomic characteristics were studied from
1992 to 1995. The results showed that the variation of grain protein content and its fractions in
somaclones from single cell culture in vitro were very significant and the range was very wide
(11531770%). Several types of variation were found in the studies, especially the type with
higher protein content than that of cultivar NE 7742 (non-culture parent). Among them, -2069% of
lines the grain protein content was significantly higher than that of NE 7742 and combined with
high yielding potential. The tendency of variation of the four protein fractions showed that the
variation of albumin was not obvious and maintained the same level as NE774 increased in
some somaclones and decreased in others. However, the percentages both globulin and
glutenin tended to increase. The variation of total amount of structural protein and the ratio
between globulin and glutenin tended to increase. The variation of total amount of structural
protein and the ratio between globulin and albumm was mainly influenced by globulin under the
condition of culture in vitro. The variation of total amount of storage protein and the ratio
between gliadin and glutenin was mainly affected by glutenin. The results mentioned above
demonstrated that the induction and screening of somaclonal variation could be an effective
way in wheat improvement in combining high protein content with high yield. |
