In vitro antiviral activity of chestnut and quebracho woods extracts against avian reovirus and metapneumovirus |
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| Institution: | 1. Dipartimento di Sanità Pubblica Veterinaria e Patologia Animale, Università di Bologna, Italy;2. Dipartimento di Sanità Pubblica, Patologia Comparata e Igiene Veterinaria, Università di Padova, Italy;3. Silvachimica, San Michele in Mondovì (CN), Italy;1. School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, 639798 Singapore, Singapore;2. Centre for Biomimetic Sensor Science, Nanyang Technological University, 50 Nanyang Drive, 637553 Singapore, Singapore;3. School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459 Singapore, Singapore;1. National Bureau of Animal Genetic Resources, GT Road By-Pass, Karnal-132001, Haryana, India;2. Immune Regulation, WPI-IFREC, Osaka University, Osaka, Japan;3. Laboratory of Molecular Biophysics, Institute of Protein Research, Osaka University, Osaka Prefecture 5650871, Japan;1. Department of Pediatrics, Peking University First Hospital, Beijing 100034, China;2. Department of Neurology, Peking University First Hospital, Beijing 100034, China;3. Lab of Electron Microscopy, Peking University First Hospital, Beijing 100034, China;1. Department of Biology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran;2. Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran 13164, Iran;3. Department of Virology, Pasteur Institute of Iran, Tehran 13164, Iran;1. College of Pharmacy, Chosun University, Gwangju, Republic of Korea;2. Department of Oral and Maxillofacial Pathology, Daejeon Dental Hospital, College of Dentistry, Wonkwang Bone Regeneration Research Institute, Wonkwang University, Daejeon, Republic of Korea |
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| Abstract: | Field evidences have suggested that a natural extract, containing tannins, could be effective against poultry enteric viral infections. Moreover previous studies have shown that vegetable tannins can have antiviral activity against human viruses. Based on this knowledge three different Chestnut (Castanea spp.) wood extracts and one Quebracho (Schinopsis spp.) wood extract, all containing tannins and currently used in the animal feed industry, were tested for in vitro antiviral activity against avian reovirus (ARV) and avian metapneumovirus (AMPV). The MTT assay was used to evaluate the 50% cytotoxic compounds concentration (CC50) on Vero cells. The antiviral properties were tested before and after the adsorption of the viruses to Vero cells. Antiviral activities were expressed as IC50 (concentration required to inhibit 50% of viral cytopathic effect). CC50s of tested compounds were >200 μg/ml. All compounds had an extracellular antiviral effect against both ARV and AMPV with IC50 values ranging from 25 to 66 μg/ml. Quebracho extract had also evident intracellular anti-ARV activity (IC50 24 μg/ml). These preliminary results suggest that the examined vegetable extracts might be good candidates in the control of some avian virus infections. Nevertheless further in vivo experiments are required to confirm these findings. |
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