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紫叶李组织培养及快繁体系的建立
引用本文:孙在红,夏阳,梁慧敏,刘荣堂.紫叶李组织培养及快繁体系的建立[J].草原与草坪,2005(1):58-61.
作者姓名:孙在红  夏阳  梁慧敏  刘荣堂
作者单位:甘肃农业大学,草业学院,甘肃,兰州,730070;山东省林业科学研究院,山东,兰州,250014
基金项目:山东省科技发展基金;国家高技术研究发展计划(863计划)
摘    要:试验以紫叶李春季萌生苗嫩枝为外植体,在腋芽诱导的基础上,建立“以芽尖诱导丛生芽”的组培快繁体系。腋芽诱导培养基为MS+6BA0.5mg/L+NAA0.1mg/L与MS+6BA1.0mg/L+NAA0.1mg/L;丛生芽增殖培养基为MS+BA1.0mg/L+IBA0.3mg/L;壮苗培养基为MS+6BA0.4mg/L+IBA0.1mg/L+GA30.2mg/L。最佳生根培养基为1/2MS+IBA1.0mg/L+蔗糖20g/L。

关 键 词:紫叶李  组织培养  丛生芽增殖  植物激素
文章编号:1009-5500(2005)01-0058-03
修稿时间:2004年6月30日

Tissue culture and rapid propagation of readleaf cherry plum in vitro
SUN Zai-hong.Tissue culture and rapid propagation of readleaf cherry plum in vitro[J].Grassland and Turf,2005(1):58-61.
Authors:SUN Zai-hong
Institution:SUN Zai-hong~
Abstract:Tissue culture and rapid propagation system of Redleaf cherry plum was established by using sprouted branch of spring as explants, base on of axillary buds inducing.Medium foraxillary buds inducing wasMS+6-BA 0.5 mg/L+NAA 0.1 mg/L and MS+6-BA 1.0 mg/L+NAA 0.1mg/L,for adventitious buds proliferation was MS+BA 1.0 mg/L+IBA 0.3 mg/L, for strong seedling was MS +6-BA 0.4 mg/L+IBA 0.1 mg/L+GA (3) 0.2 mg/L.T he optimum medium for shoot inducing was 1/2 MS+IBA 1.0 mg/L+source 20 g/L.
Keywords:Redleaf cherry plum  tissue culture  adventitious buds proliferation  phytohormone
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