| 小麦抗叶锈病近等基因系TcLr41基因的差异表达 |
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| 引用本文： | 李星,李亚宁,刘大群,杨文香.小麦抗叶锈病近等基因系TcLr41基因的差异表达[J].华北农学报,2008,23(5). |
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| 作者姓名： | 李星 李亚宁 刘大群 杨文香 |
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| 作者单位： | 河北农业大学,植物病理系,河北省农作物病虫害生物防治工程技术研究中心,河北,保定,071001 |
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| 基金项目： | 国家重大基础研究前期专项，高校博士点基金，中国博士后科学基金 |
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| 摘 要： | 为了研究与小麦抗叶锈病相关基因的表达情况,从分子水平阐明小麦的抗病机制.以Thatcher和Thatcher为遗传背景的小麦抗叶锈病近等基因系TcLr41为材料,利用cDNA-AFLP技术,开展了与小麦抗叶锈病基因Lr41相关的差异表达基因研究.共选用180对引物组合对Lr41差异表达的基因进行了分析,获得了61对能够在小麦近等基因系TcLr41和感病对照Thatcher之间扩增出差异条带的多态性引物.获得5对能够扩增出5条对小麦抗叶锈病近等基因系TcLr41具有特异性条带的引物,分别是P-AC/M-CAA、P-AG/M-CAT、P-AG/M-CCC、P-CC/M-CCA、P-GA/M-CAA.将重复性好的4条差异片段进行回收、克隆、测序,经序列同源性检索发现了1条与小麦抗叶锈病基因Lr1编码蛋白同源性较高的序列,其他的3个序列功能未知,研究结果为探明TcLr41的抗病机制和进行该抗病基因的克隆奠定了基础.
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| 关 键 词： | 小麦叶锈病 抗病基因 |
Differential Expression of Genes in Wheat Near Isogenic Line TcLr41 |
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| LI Xing,LI Ya-ning,LIU Da-qun,YANG Wen-xiang.Differential Expression of Genes in Wheat Near Isogenic Line TcLr41[J].Acta Agriculturae Boreali-Sinica,2008,23(5). |
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| Authors: | LI Xing LI Ya-ning LIU Da-qun YANG Wen-xiang |
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| Institution: | LI Xing,LI Ya-ning,LIU Da-qun,YANG Wen-xiang (Department of Plant Pathology,Agricultural University of Hebei,Biological Control Center of Plant Diseases , Plant Pests of Hebei Province,Baoding 071001,China) |
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| Abstract: | The objective of this paper is to study the resistant genes expression profile of wheat leaves and the wheat resistant mechanism to the pathogen at the molecular level.cDNA-AFLP analysis was carried out between on Thatcher and its near isogenic line TcLr41 resistant to wheat leaf rust to study the differential expression related to Lr41.A total of 180 primer combinations were screened and sixty-one of them showed the ability to produce polymorphic fragments between TcLr41 and Thatcher.Five of the 61 primer ... |
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| Keywords: | cDNA-AFLP Lr41 |
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