| Transformation of Arabidopsis thaliana via Agrobacterium tumefacience with an endochitinase gene from Trichoderma, and enhanced resistance to Sclerotinia sclerotiorum |
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| 作者姓名： | DAI Fu-ming XU Tong |
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| 作者单位： | 1. Institute of Biotechnology，Zhejiang University，Hangzhou 310029，Zhejiang，China; 2. Plant Protection Institute，Shanghai Academy of Agricultural Sciences，Shanghai 201106，China |
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| 摘 要： | Sclerotinia sclerotiorum is an important pathogen to many crops and is especially damaging to rape in China. As a model plant Arabidopsis thaliana (Col0) was transformed by spraying Agrobacterium tumefacience with Trichoderma endochitinase gene ThEn-42 at initial bud stage. Eleven seedlings (corresponding to about 0.22 percent transformation) exhibited resistance to hygromycin. The DNA fragment unique to endochitinase (ThEn-42) was amplified by Arabidopsis leaf-PCR or genomic DN…
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| 关 键 词： | 阿布属 土壤杆菌 转化 基因 木霉属 真菌 核盘菌属 抗性 |
| 文章编号： | 1008-9209(2004)04-0426-01 |
Transformation of Arabidopsis thaliana via Agrobacterium tumefacience with an endochitinase gene from Trichoderma, and enhanced resistance to Sclerotinia sclerotiorum |
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| DAI Fu-Ming,XU Tong.Transformation of Arabidopsis thaliana via Agrobacterium tumefacience with an endochitinase gene from Trichoderma, and enhanced resistance to Sclerotinia sclerotiorum[J].Journal of Zhejiang University(Agriculture & Life Sciences),2004,30(4):426-426. |
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| Authors: | DAI Fu-Ming XU Tong |
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| Institution: | 1. Institute of Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang ,China;Plant Protection Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China
2. Institute of Biotechnology, Zhejiang University, Hangzhou 310029, Zhejiang ,China |
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| Abstract: | Sclerotinia sclerotiorum is an important pathogen to many crops and is especially damaging to rape in China. As a model plant Arabidopsis thaliana (ColO) was transformed by spraying Agrobacterium tumefacience with Trichoderma endochitinase gene ThEn-42 at initial bud stage. Eleven seedlings (corresponding to about 0.22 percent transformation) exhibited resistance to hygromycin. The DNA fragment unique to endochitinase ( ThEn-42 ) was amplified by Arabidopsis leaf-PCR or genomic DNA PCR. Unfertile, dwarf and normal phenotypes appeared in the T1 generation. In addition, an enhanced resistance to S. sclerotiorum was observed. The mortality percentage (7.7% to 33.3%) in transgenic plants was significantly lower than in non-transgenic plants (86. 7%) 10 days after inoculation with the pathogen. |
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| Keywords: | Arabidopsis thaliana Trichoderma endochitinase gene resistance Sclerotinia sclerotiorum |
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