Antibody directed against Marek's disease-associated tumor surface antigen can be eluted from Marek's disease tumor cells. |
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| Authors: | H K Pradhan G C Mohanty W Y Lee B Patnaik |
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| Institution: | Division of Avian Diseases, Indian Veterinary Research Institute, Izatnagar. |
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| Abstract: | Antibody directed against Marek's disease-associated tumor surface antigen (MATSA) was eluted from tumor cells of lymphomas and peripheral blood lymphocytes that were isolated from Marek's disease virus-infected chickens. Feather follicular Marek's disease virus (MDV) antigen could not be demonstrated with this antibody by indirect immunofluorescent (IF) staining. Monoclonal antibody directed against MATSA could completely block the activity of eluted antibody and vice versa. By indirect IF staining using eluted antibody and fluorescein isothiocyanate (FITC) labelled antichicken globulin conjugate. MATSA-bearing cells were detected in MDV infected and herpes virus of turkey (HVT) vaccinated birds. Blocking of immunoglobulin molecules present on B-cells by anti-chicken globulin is critical in this test. |
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