|
|||||
|
|
| 柿花发育相关的MADS2box基因克隆与表达 | |
| 引用本文： | 丁燕,韩振海,许雪峰,李天忠. 柿花发育相关的MADS2box基因克隆与表达[J]. 园艺学报, 2007, 34(1): 39-42 |
| 作者姓名： | 丁燕 韩振海 许雪峰 李天忠 |
| 作者单位： | (中国农业大学园艺植物研究所, 北京100094) |
| 摘 要： | 以雌花型柿(Diospyros kaki) ‘阳丰’花为试材, 克隆得到一个与其花发育相关的MADS2box基因, 命名为DkMADS1, GenBank登录号为DQ412058。该基因cDNA全长1 193 bp, ORF为747 bp, 编码一个含有249个氨基酸的蛋白。DkMADS1 具保守的MADS区及半保守的K区, 与其它植物中的MADS2box蛋白有很高同源性。RT-PCR表达分析表明, 该基因在‘阳丰’花的萼片、花瓣、子房和‘禅寺丸’的雄蕊中均有表达。 |
| 关 键 词： | 柿 花 基因 克隆 表达 |
| 文章编号： | 0513-353X（2007）01-0039-04 |
| 收稿时间： | 2006-04-18 |
| 修稿时间： | 2006-04-182006-10-27 |
Molecular Cloning and Expression of Flowering-related MADS-box Gene in Diospyros kaki |
|
| DING Yan,HAN Zhen-hai,XU Xue-feng,LI Tian-zhong. Molecular Cloning and Expression of Flowering-related MADS-box Gene in Diospyros kaki[J]. Acta Horticulturae Sinica, 2007, 34(1): 39-42 | |
| Authors: | DING Yan HAN Zhen-hai XU Xue-feng LI Tian-zhong |
| Affiliation: | ( Institute of Horticultural Plants, China Agricultural University, Beijing 100094, China) |
| Abstract: | A MADS-box gene named DkMADS1 was cloned from Yangfeng persimmon flowers(GenBank accession No.DQ412058).The sequence of complete DkMADS1 cDNA gene is 1 193 bp,with an ORF encoding 249 amino acids.The deduced protein contained conservative MADS domain and semi-conservative K domain.It shared highly homology to other MADS-box proteins from different species.RT-PCR analysis showed that DkMADS1 gene was expressed in sepal,petal,ovary of Yangfeng persimmon and stamen of Zenjimaru persimmon. |
| Keywords: | Persimmon Diospyros kaki Flower Gene Cloning Expression |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《园艺学报》浏览原始摘要信息 | |
| 点击此处可从《园艺学报》下载全文 | |