| 北京荷斯坦母牛BoLA-DRB3基因外显子2的克隆与序列分析 |
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| 引用本文： | 叶素成,储明星,陈国宏,石万海,曹福存.北京荷斯坦母牛BoLA-DRB3基因外显子2的克隆与序列分析[J].安徽农业大学学报,2005,32(4):456-459. |
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| 作者姓名： | 叶素成 储明星 陈国宏 石万海 曹福存 |
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| 作者单位： | 中国农业科学院畜牧研究所 北京100094
(叶素成，储明星)，扬州大学动物科技学院 扬州225009
(陈国宏)，北京奶牛中心 北京100085
(石万海)，北京奶牛中心 北京100085(曹福存) |
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| 摘 要： | 采用PCR技术扩增出北京荷斯坦母牛BoLA-DRB3基因的外显子2的267 bp大小的片段,将该片段克隆到pGEM-T Easy质粒中,重组质粒用PCR和酶切分析进行阳性克隆鉴定,然后测定核苷酸序列,并推导其氨基酸序列.北京荷斯坦牛、蒙古牛、人类、瑞典红白花牛、蒙古绵羊、西班牙山羊之间DRB3基因外显子2的核苷酸序列同源性为80.9%～95.5%,氨基酸序列同源性为75.3%～91.0%.
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| 关 键 词： | 北京荷斯坦牛 BoLA-DRB3基因 克隆 序列分析 |
| 文章编号： | 1672-352X（2005）04-0456-04 |
| 收稿时间： | 2004-06-08 |
| 修稿时间： | 2004年6月8日 |
Cloning and Sequence Analysis of Exon 2 of BoLA-DRB3 Gene in Beijing Holstein Cows |
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| YE Su-cheng,CHU Ming-xing,CHEN Guo-hong,SHI Wan-hal,CAO Fu-cun.Cloning and Sequence Analysis of Exon 2 of BoLA-DRB3 Gene in Beijing Holstein Cows[J].Journal of Anhui Agricultural University,2005,32(4):456-459. |
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| Authors: | YE Su-cheng CHU Ming-xing CHEN Guo-hong SHI Wan-hal CAO Fu-cun |
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| Institution: | 1. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094; 2. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009; 3. Beijing Dairy Cattle Centre, Beijing 100085 |
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| Abstract: | The 267 bp fragment of exon 2 of BoLADRB3 gene was amplified successfully by PCR and cloned into pGEM-T Easy vector.The positive clone was further identified by PCR and restriction enzyme analysis.The nucleotide sequence was detected and the peptide sequence of this fragment was deduced.Homologies of nucleotide sequence and amino acid sequence of exon 2 of DRB3 gene among Beijing Holstein cattle,Mongolian cattle,human,Swedish Red and White cattle,Mongolian sheep and Spanish goat were from 80.9% to 95.5% and from 75.3% to 91.0%,respectively. |
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| Keywords: | Beijing Holstein cattle BoLA-DRB3 gene cloning sequence analysis |
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