枯草杆菌果聚糖蔗糖酶基因转化小麦的研究

 【目的】尝试将枯草杆菌果聚糖蔗糖酶基因（SacB）导入小麦品种（系）02-371、02-207、郑麦9023和东农7742以提高其耐旱性。【方法】利用农杆菌介导法转化小麦幼胚愈伤组织，将SacB基因导入4个小麦品种，并对转化植株进行PCR和Southern杂交检测。【结果】SacB基因已整合到受体小麦的基因组中。转基因植株整合目的基因的拷贝数多为1～2个。4个受体小麦品种（系）02-371、02-207、郑麦9023和东农7742的转化频率分别为0.88%、1.48%、1.04%和1.23%。RT-PCR检测结果表明，SacB基因在部分转基因植株中得到表达，并使转基因小麦植株的耐干旱胁迫能力明显提高。【结论】向小麦中导入枯草杆菌SacB基因可增强其耐旱性。

Study on Transformation of Wheat with SacB Gene From Bacillus subtilis

Objective] SacB gene from Bacillus subtilis will be transferred into wheat cultivars or breeding lines 02-371, 02-207, Zhengmai 9023 and Dongnong 7742 for improving their tolerance to drought stress. Method] Using wheat immature embryo calli as explants, SacB gene was transferred into four wheat cultivars by Agmbacterium-metfiated transformation. Transformed plants were detected by PCR and Southern blotting. Result ] PCR and Southern blotting analysis showed that the SacB gene has been integrated into wheat genome. One or two copies of integrated transgene were in transgenic plants. The transformation frequencies of Line 02-371, Line 02-207, Zhengmai 9023 and Dongnong 7742 were 0.88%, 1.48%, 1.04%, and 1.23%, respectively. RT-PCR analysis showed that the SacB gene was expressed in some transgenic plants, of which the tolerance to drought stress was significantly improved. Conclusion] SacB gene from Bacillus subtilis can improve the tolerance of transgenic wheat plants to drought stress.