MALDI‐MS Lipid Profiles of Oocytes Recovered by Ovum Pickup from Bos indicus and 1/2 indicus × taurus with High vs Low Oocyte Yields |
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| Authors: | KC Silva‐Santos CR Ferreira GMG Santos MN Eberlin LS Siloto CO Rosa TN Marcantonio MM Seneda |
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| Institution: | 1. Laboratório de Biotecnologia da Reprodu??o Animal (ReproA), DCV‐CCA‐Universidade Estadual de Londrina (UEL), , Londrina, Brazil;2. ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, University of Campinas, , Londrina, Brazil |
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| Abstract: | The aim of the present study was to compare the lipid profile in oocytes of indicus and 1/2 indicus × taurus cows with high and low antral follicle count (AFC)/oocyte yields. After an OPU procedure (D0), antral follicles ≥3 mm were counted by ultrasonography (D4, 19, 34, 49, 64), and cows were assigned to groups with either high AFC (≥30 follicles; indicus, NH group; 1/2 indicus × taurus, AH group) or low AFC (≤15 antral follicles; indicus, NL group; 1/2 indicus × taurus, AL group). The lipid profiles of the oocytes were determined by MALDI‐MS. For GI, GII and GIII oocytes, the indicus samples tend to cluster separately from the 1/2 indicus × taurus samples. The lipid species PC (P‐38:5) + H]+ and/or PC (P‐36:2) + Na]+, PC (38:2) + H]+, PC (38:5) + Na]+ and TAG (60:8) + NH4]+ were more abundant in indicus (NH and NL groups) than 1/2 indicus × taurus. The higher lipid content in the indicus oocytes likely reflects differences in the rate of lipid metabolism and may contribute to oocyte competence and embryo development. |
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