Review of doubled haploid production in durum and common wheat through wheat × maize hybridization |
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Authors: | Zhixia Niu Aixiang Jiang Wesam Abu Hammad Atena Oladzadabbasabadi Steven S Xu Mohamed Mergoum Elias M Elias |
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Institution: | 1. United States Department of Agriculture, Agricultural Research Service, Cereal Crops Research Unit, , Fargo, ND, 58102 USA;2. Biostatistics Department, Vanderbilt University, , Nashville, TN, 37232 USA;3. Department of Plant Sciences, North Dakota State University, , Fargo, ND, 58108 USA |
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Abstract: | Production of doubled haploids (DHs) is an important methodology to speed the process of breeding and development of mapping populations in crops. The procedure for DH production includes two major steps: haploid induction and chromosome doubling. In recent years, wide hybridization between wheat and maize has become a main approach for haploid production in wheat. In this method, the maize chromosomes are completely eliminated during the early development of the hybrid seeds after wheat spikes were pollinated with maize pollen. Numerous wheat cultivars and mapping populations have been developed using wheat–maize hybridization. In this study, we review the procedures of DH production of durum and common wheat via wide hybridization with maize, the factors which affect the efficiency of DH production, and the mechanism of selective elimination of the maize genome during the early development of the hybrid embryos. We also report a highly efficient protocol for DH production in durum and common wheat, which was established based on the optimal conditions for each of the factors that affect the efficiency of DH production. |
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Keywords: | doubled haploid production wide hybridization chromosome elimination chromosome doubling common wheat durum wheat |
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