胡黄连单体ScrocaffesideA对小鼠细胞因子分泌影响的体外研究

目的]研究ScrocaffesideA（SA）免疫调节活性，为新型免疫增强剂的开发提供试验依据。方法]从西藏胡黄连的根茎中分离鉴定得到的单体E-咖啡酰基-6-（4-O-β—D一吡喃葡萄糖基）-E一咖啡酰基-O-β—D一吡喃葡萄糖苷，命名为ScrocaffesideA（SA）。通过用不同浓度的SA协同ConA体外刺激小鼠脾淋巴细胞，采用双抗夹心ELISA法，检测细胞上清中细胞因子的分泌量。结果]不同浓度SA协同ConA（5mg／L）与细胞作用24及48h后，在25和125mg／L浓度组显著上调了Th1细胞分泌的主要细胞因子IL-2、IL一12和IFN一1的分泌量；Th2细胞分泌的主要细胞因子IL-4和IL—10分泌量也显著升高，仅IL一10的分泌量在48h，SA浓度为125ms／L剂量时有所下降。结论]SA能上调相关细胞因子的表达，具有免疫增强作用。

Effect of Scrocaffeside A Monomer from Picrorhiza scrophulariiflora on Mouse Cytokines in Vitro

Objective] The experimental basis of the development of new type of immune agent was provided through the study on the activity of immune regulator of scrocaffeside A(SA). Method] The monomer: E-caffeoyl-6-(4-O-β-D-glucopyranosyl)-E-caffeoyl-O-β-D-pyrazole furans glucopyranoside isolated from the roots of Picrorhiza scrophulariiflora , was named as scrocaffeside A(SA) after its identification. The cytokine in the cell supernatants of the mouse, stimulatively in vitro treated by the different concentrations of SA coopered with Con A ion was tested with the method of double-antibody ELISA. Results] The cytokines of IL-2, IL-12 and IFN-γ in Th1 cells were significantly increased in the treatment of 25 mg/L and 125 mg/L, which reaction between the different concentrations of SA coopered with Con A(5mg/L) and cells lasted 24 and 48 hours and the cytokines of IL-4 and IL-10 in Th2 cell was also significantly increased, only IL-10 was declined in the treatment condition of the reaction time, 48 hours and the concentration of SA, 125 mg/L. Conclusion] SA could strengthen the expression of cytokines associated with stronger immune function.