Genetic Diversity of Cultivated Chinese Chive Germplasm by ISSR and RAPD Markers

利用RAPD(Random Amplified Polymorphic DNA)和ISSR(Inter-simple Sequence Repeat)两种分子标记技术对20份韭菜栽培品种进行了遗传多样性研究。结果表明，筛选后选用的12个ISSR引物和15个RAPD引物分析分别产生了258和101条扩增产物带，其中多态性条带(即20个韭菜品种中一个或多个但不是全部具有的带)分别为132和40条，分别占总数的51.2%和39.6%。，也就是说12个ISSR引物和15个SSR引物对韭菜不同品种的扩增可分别产生51

Genetic Diversity of Cultivated Chinese Chive Germplasm by ISSR and RAPD Markers

Random amplified polymorphic DNA (RAPD) and Inter-simple sequence repeat (ISSR) molecular markers were used to analyze the genetic diversity of 20 cultivated Chinese chive's germplasm. The results are as fellows: Of the primers tested, 12 ISSR and 15 RAPD primers were selected for their reproducibility and high polymorphism. About 258 bands were obtained by 12 ISSR primers and 101 bands were obtained by 15 RAPD primers. Of which , the polymorphic bands are 132 and 40 ,the percent are 51.2 % and 39.6 % respectively. The data were analyzed by NYSTS program. Based on the data, the Nei's genetic distance is obtained by NTSYS program, then UPCMA cluster analysis is using by the Nei's genetic distance . The conclusion is that the tested materials have the inferior genetic diversity , and the genetic distance is 0.02-0.2 and 0.04-0.13 among the breeds respectively. Most of the materials aren't clustering by the provinces. Whereas , based on the correlation test gained by Mantel Test (1968) through analyzing the genetic diversity ,we can found that the two results have the lower correlation ;r=0.47174, P<0.05.