Cloning and Characterization of Full Length cDNA of a CC-NBS-LRR Resistance Gene in Sweetpotato |
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Authors: | CHEN Guan-shui ZHOU Yi-fei HOU Li-li PAN Da-ren |
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Institution: | 1. College of Life Science,Fujian Agriculture and Forestry University,Fuzhou 350002,P.R.China 2. College of Crop Science,Fujian Agriculture and Forestry University,Fuzhou 350002,P.R.China |
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Abstract: | Conserved domain such as nucleotide binding site (NBS) was found in several cloned plant disease resistance genes.Based on the NBS domain,resistance gene analogues (RGAs) have been isolated.A full-length cDNA,SPRI was obtained by rapid amplification of cDNA ends (RACE) method.Sequence analysis indicated that the length of SPR1 was 3 066 bp,including a complete open reading frame of 2 667 bp encoding SPRI protein of 888 amino acids.Compared with known NBS-LRR genes,it presented relatively high amino acid sequence identity.The polypeptide has a typical structure of non TIR-NBS-LRR genes,with NB-ARC,CC,and LRR domains.The SPR1-related sequences belonged to multicopy gene family in sweetpotato genome according to the result of Southern blotting.Semi-quantitative RT-PCR analysis showed SPR1 expressed in all tested tissues.The cloning of putative resistance gene from sweetpotato provides a basis for studying the structure and function of sweetpotato disease-resistance relating genes and disease resistant genetic breeding in sweetpotato.The gene has been submitted to the GenBank database,and the accession number is EF428453. |
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Keywords: | sweetpotato NBS (nucleotide binding site) LRR (leucine-rich repeat) R gene (resistance gene) |
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