| 淡豆豉中异黄酮提取工艺的优化研究 |
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| 引用本文： | 牛丽颖,蔡广华,刘敏彦,王鑫国.淡豆豉中异黄酮提取工艺的优化研究[J].大豆科学,2011,30(1):164-167. |
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| 作者姓名： | 牛丽颖 蔡广华 刘敏彦 王鑫国 |
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| 作者单位： | 牛丽颖,蔡广华,王鑫国,NIU Li-ying,CAI Guang-hua,WANG Xin-guo(河北医科大学中医学院,河北,石家庄,050091);刘敏彦,LIU Min-yan(河北以岭医药研究院有限公司,河北,石家庄,050035) |
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| 摘 要： | 采用单因素和正交试验相结合的方法,以淡豆豉总异黄酮和染料木素的提取量为评价指标,优选淡豆豉中异黄酮的提取工艺.结果表明淡豆豉中提取异黄酮的最佳工艺为:淡豆豉药材8倍量70%乙醇回流提取3次,每次2h.
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| 关 键 词： | 淡豆豉 异黄酮 染料木素 UV HPLC |
Research on Optimization of Isoflavone Extracting Technique from Semen Sojae Praeparatum |
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| NIU Li-ying,CAI Guang-hua,LIU Min-yan,WANG Xin-guo.Research on Optimization of Isoflavone Extracting Technique from Semen Sojae Praeparatum[J].Soybean Science,2011,30(1):164-167. |
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| Authors: | NIU Li-ying CAI Guang-hua LIU Min-yan WANG Xin-guo |
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| Institution: | NIU Li-ying1,CAI Guang-hua1,LIU Min-yan2,WANG Xin-guo1(1.Traditional Chinese Medical College,Hebei Medical University,Shijiazhuang 050091,2.Institute of Hebei Yiling Pharmaceutical Co.,Ltd,Shijiazhuang 050035,Hebei,China) |
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| Abstract: | The best extracting technique of Semen Sojae Paratum(SSP) were optimized by orthogonal test.The extracting contents of total isoflavone and genistein in SSP were determined by UV detector and HPLC,respectively.The results showed that the best technological process of isoflavone extraction was to reflux SSP by 70% ethanol 3 times and 2 hours for each time.The content of 70% ethanol was 8 times of SSP. |
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| Keywords: | UV HPLC |
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