福建地区2株猪嵴病毒 VP1基因的克隆及遗传进化分析

目的]调查猪嵴病毒在福建地区腹泻猪群中的流行和变异情况。方法]根据Gen Bank中登陆的猪嵴病毒(porcine kobuvirus,PKo V)结构蛋白VP1基因序列设计特异性引物,采用RT-PCR方法从某猪场采集腹泻小肠样品中扩增猪嵴病毒VP1基因,将扩增后的目的片段克隆后进行序列测定。应用生物信息学软件,将获得的2株猪嵴病毒VP1和Gen Bank的猪嵴病毒株VP1基因序列进行对比分析。结果]猪嵴病毒CH/FJNP/12L/2015与匈牙利株K-30-HU/2008/HUN(GQ249161)的核苷酸同源性最高,为88.1%,氨基酸同源性为95.3%。CH/FJNP/12W1/2015与越南株714441/CAOLANH-VH/2012-2-21(KT266058)的核苷酸同源性最高,为88.2%,氨基酸同源性为96.1%,同源重组分析显示,2株毒株均无明显同源重组发生。结论]频繁的畜禽国际贸易,以及如今便捷的现代化交通工具,人们生活提供方便的同时,也加速了猪嵴病毒毒性的传播。

Cloning of Porcine Kobuvirus VP1 in Fujian province and Phylogenetic analysis

Objective] The current situation of the epidemic and genetic variation of the porcine Kobuvirus (PKV) in diarrhea pigs in Fujian province were investigated.Method] The special primer was designed according to the sequence of PKV deposited in GenBank.The VP1 gene of PKV in intestinal samples collected from pig -raising farm was amplified with RT -PCR method, and then, the amplified target frag-ment was cloned and sequenced.The sequences of two PKV strains were analyzed and compared by means of bioinformatics software.Re-sults] The results showed that there was high nucleotide homology between VP1 gene of CH /FJNP/12L /2015and K -30 -HU /2008 /HUN, which was 88.1%; amino acid homology, 95.3%.The homology in the nucleotide and amino acid of CH /FJNP/12W1 /2015 with 714441 /CAOLANH -VH /2012 -2 -21 was 88.2% and 96.1%, respectively.The two isolates had no obvious homologus recombination occurred with other strains.Conclusion] speculated that the frequent international livestock trade, and is now a modern and convenient means of trans-port, in convenience to our lives, but also accelerate the spread of swine crest virus strains.Conclusion] It is speculated that it resulted from the frequent international trade of livestock and poultry, and convenient modern transportation tools, which also accelerated the transmission of PKV.