不同检测仪器对SD大鼠血液生理指标测定的影响

目的：比较实验动物专用血细胞分析仪与医用血细胞分析仪对SD大鼠血液生理指标测定值的差异，分析检测仪器对大鼠血液生理指标测定的影响。方法：36只SD大鼠，体重约200 g，雌雄各半，屏障环境饲养，试验前禁食12 h，麻醉后采血，分别用实验动物专用血细胞分析仪与医用血细胞分析仪测定20项血液生理指标。结果：两种仪器分别检测的SD大鼠的平均血红蛋白浓度、平均血红蛋白含量、白细胞数量、淋巴细胞数量、单核细胞数量、嗜碱性粒细胞数量、嗜中性白细胞百分比、单核细胞百分比、嗜酸性粒细胞百分比、嗜碱性粒细胞百分比、淋巴细胞百分比、血小板数量、血小板分布宽度差异极显著（P＜0.01）；血红蛋白浓度、嗜中性白细胞数量、红细胞分布宽度差异显著（P＜0.05）；红细胞数量、红细胞压积、平均红细胞体积、嗜酸性粒细胞数量差异不显著（P＞0.05）。结论：不同检测仪器对SD大鼠血液学指标的检测有影响。

Effects on blood physiological indexes of SD rats from different detecting instruments

Objective The purpose of the study was to investigate the level of blood physiological values for SD rats between special ex-perimental animal blood cell analyzer and medical blood cell analyzer. Methods 200 g weight SD rats (18 male and 18 female) were chosen and fed in SPF environment, which fasted before experiment and blooded after anesthesia. The blood samples were collected and tested for physiological values using special experimental animal blood cell analyzer and medical blood cell analyzer, and the re-sults of two groups were compared.Results There were extremely significant differences in HGB, MCH, WBC, LYMP, MONO, BASO, NEUT%, MONO%, EOS%, BASO%, LYMP%, PLT and PDW of SD rats between special experimental animal blood cell analyzer and medical blood cell analyzer (P<0.01). There were significant differences in MCHC, NEUT and RDW of SD rats between special ex-perimental animal blood cell analyzer and medical blood cell analyzer (P<0.01). However, there were not significant differences in RBC, HCT, MCV and EOS (P>0.05). Conclusions Thus, the results suggested that different detecting instruments could influence some blood physiological values of SD rats.