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马铃薯Y病毒黑龙江分离物株系鉴定
引用本文:高秀妍,高艳玲,耿宏伟,张威,范国权,白艳菊,吕文河,蒋希峰. 马铃薯Y病毒黑龙江分离物株系鉴定[J]. 中国马铃薯, 2009, 23(1)
作者姓名:高秀妍  高艳玲  耿宏伟  张威  范国权  白艳菊  吕文河  蒋希峰
作者单位:1. 东北农业大学,黑龙江,哈尔滨,150030;黑龙江省农业科学院,黑龙江,哈尔滨,150086
2. 黑龙江省农业科学院,黑龙江,哈尔滨,150086
3. 东北农业大学,黑龙江,哈尔滨,150030
摘    要:通过生物学方法、血清学方法、RT-PCR方法对黑龙江省一个马铃薯Y病毒分离物进行株系鉴定。该病毒侵染洋酸桨产生枯斑,在黄苗榆烟上表现系统花叶,并伴有坏死症状,血清学反应强烈,三重PCR特异性条带清晰,鉴定该分离物为PVYN。

关 键 词:PVYN  生物学  血清学  RT-PCR  花叶  坏死

Identification of a Potato Virus Y Isolate from Heilongjiang
Gao Xiuyan,Gao Yanling,Geng Hongwei,Zhang Wei,Fan Cuoquan,Bai Yanju,Lu Wenhe,Jiang Xifeng. Identification of a Potato Virus Y Isolate from Heilongjiang[J]. Chinese Potato, 2009, 23(1)
Authors:Gao Xiuyan  Gao Yanling  Geng Hongwei  Zhang Wei  Fan Cuoquan  Bai Yanju  Lu Wenhe  Jiang Xifeng
Affiliation:1.Northeast Agricultural University;Harbin;Heilongjiang 150030;China;2.Heilongjiang Academy of Agricultural Sciences;Heilongjiang 150086;China
Abstract:A Potato Virus Y isolate collected from Heilongjiang was identified using host plants,serology and RT-PCR.The virus could infect Physalis floridana and produce necrotic ringspot.Serological reaction to this virus was very strong.Specific bands were obvious when the isolate was tested by Multiplex RT-PCR.Therefore,the isolate was considered to be PVYN.
Keywords:PVYN  RT-PCR
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