小菘菜游离小孢子培养技术研究

以4个引自日本的小菘菜杂交种为试材进行小孢子培养，对影响小菘菜胚状体发生及其成苗的因素进行分析。结果表明：通过游离小孢子培养，获得了大量的小孢子胚状体及再生植株85株；不同基因型间的小孢子胚诱导率差异显著；4 ℃低温预处理24 h对小孢子胚的发生具有促进作用；NLN-13+0.2 mg?L-1 6-BA+0.1 mg?L-1 NAA为适宜的诱胚培养基；50 r?min-1低频振荡培养有利于提高子叶形胚发生的比例；MS+3 %蔗糖+0.75 %琼脂+0.1 g?L-1活性炭是小孢子植株继代和壮苗的适宜培养基；MS+3 %蔗糖+ 0.75 %琼脂+0.1 mg?L-1 NAA是小孢子植株适宜的生根培养基。

Studies on Isolated Microspore Culture in [ Brassica campestris L.ssp.chinensis ( L.) Makino var.communis Tsen et Lee ]

This study is to establish a stable and efficient system of isolated microspore culture in〔Brassica campestris L. ssp. chinensis（L.）Makino var. communis Tsen et Lee〕. The key factors influencing embryogenesis and plant regeneration were studied with 4 hybrid varieties introduced from Japan. Embryoids and 85 regenerated plants were obtained. Results showed that genotypes had extremely significant effect on frequency of embryogenesis. Low temperature pretreatment at 4 ℃ for 24 h could enhance embryo frequencies. NLN-13+0.2 mg?L-1 6-BA+0.1 mg?L-1 NAA could improve the frequency of microspore embryogenesis. Liquid culture in condition of 50 r?min-1 low-frequency oscillation could increase the proportion of cotyledon-shaped embryoids. The best subculture and rejuvenation medium was MS+3 % sucrose+0.75 % agar+0.1 g?L-1 activated charcoal. MS+3 % sucrose+0.75 % agar+0.1 mg?L-1 NAA was a kind of suitable medium for the rooting of microspore plantlets.