核盘菌侵染拟南芥的过程及其芥子油苷的变化

研究了核盘茵侵染拟南芥哥伦比亚生态型(Col-O)过程中,拟南芥的发病症状、活性氧和芥子油苷组分和含量的变化,及其抗性相关基因和芥子油苷生物合成相关基因表达的变化.结果表明,在侵染过程中,拟南芥的受侵染部位出现黄色水渍状病斑,随发病时间的延长病斑逐渐扩大到整株,其相对含水量逐渐下降,相对电导率不断上升,活性氧(过氧化氢...

GLUCOSINOLATE CHANGES IN Arabidopsis thaliana Infected By Sclerotinia sclerotiorum

The symptoms and changes in the levels of reactive oxygen species (ROS) and glucosinolates, were studied in the course of Sclerotinia sclerotiorum infection of Arabidopsis thaliana Columbia (Col-0) ecotype as well as the expression of resistance related genes and glucosinolate biosynthesis related genes.Results showed that during the infection, yellow lesions appeared from the infection sites, and gradually expanded to the entire plant, causing lethal damage. The relative water content decreased gradually, while the relative electric conductivity increased, and ROS (hydrogen peroxide and superoxide anion) accumulated steadily, leading to plant cell death. The contents of glucoraphanin, total aliphatic glucosinolates, and total glucosinolates declined, but the contents of glucobrassicin, 4-methoxyglucobrassicin and total indole glucosinolates increased at the beginning and then decreased. The expression of resistance related genes and indole glucosinolates related genes were greatly enhanced. The induction of resistance was partially systemic. These results suggest that glucosinolates, especially indole glucosinolates might be involved in the defense of Arabidopsis against S. sclerotiorum.