毛豆中啶虫脒的残留分析方法

建立了用高效液相色谱法测定毛豆中啶虫脒残留量的方法。试样用二氯甲烷超声波提取,中性氧化铝柱净化,石油醚去除脂类杂质,以0.2%冰乙酸水溶液 乙腈=70 30,(V/V)为流动相,配备AgilentTC-C18柱、高效液相色谱紫外检测器检测,外标法定量。实验表明,毛豆样品中啶虫脒添加回收率在88.0%～103.5%之间,相对标准偏差(RSD,n=3)在7.5%-11.2%之间,啶虫脒在毛豆样品中的最低检出限为0.016mg/kg。

Determination of Acetamiprid Residue in Vegetable Soybean

A HPLC method for the determination of acetamiprid residue in vegetable soybean was established. The vegetable soybean sample was extracted using dichloromethane. Aluminium oxide chromatographic column was used to clean up with petroleum ether. The HPLC conditions were as follows: Agilent TC-C18 column,(0.2% acetic acid solution) acetonitrile=70 30(V/V)as mobile phase,and UV detection at 270nm. The recoveries of acetamiprid in vegetable soybean samples were ranged from 88.0% to 103.5%,relative standard deviation(RSD,n=3)were ranged from 7.5% to 11.2%,and the minimun limit of detection of acetamiprid in vegetable soybean samples was 0.016mg/kg.