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| 可视化LAMP快速检测猪伪狂犬病毒 | |
| 引用本文： | 王树芬,王德国,薛白,李学伍,赵东,张改平. 可视化LAMP快速检测猪伪狂犬病毒[J]. 河南农业科学, 2012, 41(6): 140-143 |
| 作者姓名： | 王树芬 王德国 薛白 李学伍 赵东 张改平 |
| 作者单位： | 1. 河南科技大学动物科技学院,河南洛阳471003;河南省农业科学院农业部动物免疫学重点开放实验室/河南省动物免疫学重点实验室,河南郑州450002 2. 许昌学院食品科学与工程学院,河南许昌,461000 3. 河南科技大学动物科技学院,河南洛阳,471003 4. 河南省农业科学院农业部动物免疫学重点开放实验室/河南省动物免疫学重点实验室,河南郑州,450002 |
| 基金项目： | 现代农业产业技术体系建设专项资金资助项目(nycytx 009) |
| 摘 要： | 为快速检测猪伪狂犬病毒(PRV),利用PRV DBP基因上的一段序列设计并合成3对LAMP引物,采用一种新的荧光染料——罗丹明B衍生物作指示剂,该指示剂在反应前加到反应缓冲液里,通过优化反应条件,建立了可视化LAMP快速检测PRV的方法。结果表明,63℃下恒温反应40min可得到肉眼可视结果,颜色变成蓝色判定为阳性,仍然保持紫色判定为阴性,可视化LAMP结果与电泳结果一致。建立的可视化LAMP方法可以快速、直观、准确地检测PRV。 |
| 关 键 词： | 猪伪狂犬病毒(PRV) 环介导等温扩增技术(LAMP) 罗丹明B衍生物指示剂 |
Establishment of a Visuable LAMP for the Rapid Detection of Pseudorabies Virus |
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| WANG Shu-fen , WANG De-guo , XUE Bai , LI Xue-wu , ZHAO Dong , ZHANG Gai-ping. Establishment of a Visuable LAMP for the Rapid Detection of Pseudorabies Virus[J]. Journal of Henan Agricultural Sciences, 2012, 41(6): 140-143 | |
| Authors: | WANG Shu-fen WANG De-guo XUE Bai LI Xue-wu ZHAO Dong ZHANG Gai-ping |
| Affiliation: | 1.College of Animal Science and Technology,Henan Science and Technology University,Luoyang 471003,China; 2.Key Laboratory of Animal Immunology of the Ministry of Agriculture/Henan Provincial Key Laboratory of Animal Immunology,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China; 3.College of Food Science and Enginering,Xuchang University,Xuchang 461000,China) |
| Abstract: | Three pairs of primers were designed according to conservative sequences on DBP genes on Pseudorabies virus(PRV).A method of loop-mediated isothermal amplification(LAMP) for rapid and visible detection of PRV has been established by optimizing reacting system using rhodamine B derivatives as the indicator.The results showed that the visible results can be observed at 40 min under the condition of 63 ℃ water bath.The blue color is regarded as positive while the purple is negative.The result of visible LAMP was in accordant with that of agarose gel electrophoresis. |
| Keywords: | Pseudorabies virus(PRV) loop-mediated isothermal amplification(LAMP) Rhodamine B derivatives |
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