| 黔东南小香羊GDF9基因遗传变异分析 |
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| 引用本文： | 蔡惠芬,任珍珍,杨忠诚,罗卫星,刘若余.黔东南小香羊GDF9基因遗传变异分析[J].贵州大学学报(农业与生物科学版),2010,29(4):371-373. |
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| 作者姓名： | 蔡惠芬 任珍珍 杨忠诚 罗卫星 刘若余 |
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| 作者单位： | 1. 贵州省畜牧技术推广站,贵州,贵阳,550001
2. 贵州大学,高原山地动物遗传育种与繁殖省部共建教育部重点实验室,贵州,贵阳,550025 |
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| 基金项目： | 贵州省畜牧技术推广站资助项目 |
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| 摘 要： | 采用直接测序法检测GDF9基因在黔东南小香羊群体中的单核苷酸多态性。结果表明：在GDF9基因外显子2的550位碱基处发生了突变（A→C）并导致组氨酸突变为脯氨酸；试验检测到AA型、Aa型和aa型3种基因型；A等位基因频率为0．6667，a等位基因频率为0．3334。
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| 关 键 词： | GDF9基因 遗传变异 黔东南小香羊 |
Genetic Variation in GDF9 Gene of Small Xiang Goats in Southeast Guizhou |
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| CAI Hui-fen,REN Zhen-zhen,YANG Zhong-cheng,LUO Wei-xing,LIU Ruo-yu.Genetic Variation in GDF9 Gene of Small Xiang Goats in Southeast Guizhou[J].Journal of Mountain Agriculture & Biology,2010,29(4):371-373. |
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| Authors: | CAI Hui-fen REN Zhen-zhen YANG Zhong-cheng LUO Wei-xing LIU Ruo-yu |
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| Institution: | 1. Ministry of Education Key Laboratory for Animal Genetics, Breeding and Reproduction, Guizhou University, Guiyang Guizhou 550025, China; 2. Animal Husbandry Technology Extension Station of Guizhou ,Guiyang Guizhou 550001, China) |
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| Abstract: | Single nucleotide polymorphism of growth differentiation factor 9 （GDF9） gene, a candidate gene on the prolificacy, was revealed by sequencing in Small Xiang Goats of Southeast Guizhou in the present study. The results showed that there was one single nucleotide mutation （A^550→C^550） at exon 2 of this gene in Small Xiang Goats, which resulted in an amino acid change： histidine→proline. Therefore, three genotypes （AA , Aa and aa） were detected in Small Xiang Goats. The frequencies of alleles A and a were 0. 666 7and 0. 333 4, respectively |
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| Keywords: | Growth differentiation factor 9 gene genetic variation Small Xiang Goats of Southeast Guizhou |
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