Characterization of Longidorus helveticus (Nematoda: Longidoridae) from the Czech Republic |
| |
| Authors: | Shesh Kumari Sergei A Subbotin |
| |
| Institution: | 1. Division of Plant Health, Crop Research Institute, Drnovská 507, Ruzyně, 16106, Prague 6, Czech Republic
2. California Department of Food and Agriculture, Plant Pest Diagnostics Center, 3294 Meadowview Road, Sacramento, CA, 95832-1448, USA
|
| |
| Abstract: | Longidorus helveticus was found at two out of 285 sampling sites for the first time in the Czech Republic. Females, males and juvenile stages were analyzed morphologically and morphometrically. The morphological identification of samples was verified by polymerase chain reaction using a species specific primer. Four markers of ribosomal DNA (18S, ITS1, ITS2, D2-D3 expansion segments of 28S rRNA) and two markers of mitochondrial DNA (cox1 and nad4) were sequenced and analyzed and compared with published gene sequences of other populations of L. helveticus. The partial mitochondrial cytochrome c-oxidase subunit 1 gene and partial nicotinamide dehydrogenase subunits 4 gene showed relatively high genetic variation within the species compared with ribosomal DNA markers. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
