| 猪Ob-Rb基因的克隆与分析 |
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| 引用本文： | 朱达文,周春宝,倪黎纲,韩大勇.猪Ob-Rb基因的克隆与分析[J].安徽农业科学,2009,37(35):17404-17405. |
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| 作者姓名： | 朱达文 周春宝 倪黎纲 韩大勇 |
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| 作者单位： | 江苏畜牧兽医职业技术学院,江苏泰州,225300;江苏畜牧兽医职业技术学院,江苏泰州,225300;江苏姜曲海种猪场,江苏泰州,225300 |
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| 摘 要： | 目的]采用RT-PCR法克隆与分析瘦素受体06-Rb cDNA序列。方法]从160日龄初情期的苏姜猪的下丘脑中提取组织总RNA．根据GenBank中猪06．RbmRNA全序列设计引物，用反转录-聚合酶链式反应（RT—PCR）进行cDNA扩增，获得1条343bp的片段，将PCR产物克隆于pGEM—Teasy载体后进行测序分析。结果]用紫外分光光度计检测所提取的RNA质量，0D260／OD280=1.9，证明所提RNA的质量较好，无降解和蛋白质污染；用1％的琼脂糖检测所提RNA，有3条清晰的条带，分别是28S、18S、5S，28S与18S浓度比约为2：1．说明所提的RNA的完整性较好。所获D6-RbcDNA序列用Blast程序与在GenBank中发表的猪06-Rb cDNA序列（AF092422）比较表明，其同源性为100％，说明所扩增的序列是正确的。结论]该研究为进一步探索Ob-Rb基因组织表达和作用机理奠定了理论基础。
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| 关 键 词： | 猪 Ob-Rb基因 克隆 序列分析 |
Cloning of Ob-Rb Gene of Porcine and Its Sequence Analysis |
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| ZHU Da-wen et al.Cloning of Ob-Rb Gene of Porcine and Its Sequence Analysis[J].Journal of Anhui Agricultural Sciences,2009,37(35):17404-17405. |
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| Authors: | ZHU Da-wen |
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| Institution: | ZHU Da-wen et al(Jiangsu Vocational Technical College of Animal Husb,ry , Veterinary,Taizhou,Jiangsu 225300) |
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| Abstract: | Objective] The study aimed to clone and analyze the Ob-Rb cDNA sequence of leptin receptor by using RT-PCR method.Method] The total RNA of porcine was extracted from hypothalamus of 160-day-old Sujiang pig at puberty.A pair of primers was designed according to the Ob-Rb mRNA whole sequence reported in GenBank previously.A cDNA fragment in length 343 bp was synthesized and amplified by RT-PCR.The PCR product was cloned into pGEM-T easy vector and then sequenced.Result] When the extracted RNA was detected ... |
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| Keywords: | Pig Ob-Rb gene Cloning Sequence analysis |
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