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| 黄粉虫不同发育时期酚氧化酶活性及表达差异研究 | |
| 引用本文： | 吴必顺,韩宏岩,王大永,宋宜娟,马洪利,许维岸. 黄粉虫不同发育时期酚氧化酶活性及表达差异研究[J]. 安徽农业科学, 2010, 38(17): 9041-9042 |
| 作者姓名： | 吴必顺 韩宏岩 王大永 宋宜娟 马洪利 许维岸 |
| 作者单位： | 苏州大学基础医学与生物科学学院,江苏苏州,215123 |
| 摘 要： | [目的]研究黄粉虫不同发育时期酚氧化酶（PO）的活性及其酶原（PPO）mRNA表达量差异。[方法]用3种不同的处理方式处理黄粉虫末龄幼虫、蛹和成虫。24 h后测定血淋巴的PO活性,并通过半定量RT-PCR方法检测PPO的表达量。[结果]PO活性表现出随龄期推进活性加强的趋势,即成虫的活性最高,蛹次之,末龄幼虫最低。没有经过处理时,蛹期几乎没有PPO基因的表达。经细菌处理后,PPO表达量增加,但末龄幼虫最低。[结论]黄粉虫不同发育时期的血淋巴PO活性以及表达量存在差异,具有发育时期相关性。 |
| 关 键 词： | 酚氧化酶 黄粉虫 半定量RT-PCR |
Study on the Differences of PO Activity and the mRNA Expression during Different Developmental Stages of Tenebrio molitor |
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| WU Bi-shun et al. Study on the Differences of PO Activity and the mRNA Expression during Different Developmental Stages of Tenebrio molitor[J]. Journal of Anhui Agricultural Sciences, 2010, 38(17): 9041-9042 | |
| Authors: | WU Bi-shun et al |
| Affiliation: | WU Bi-shun et al(College of Preclinical Medicine and Biological Science,Soochow University,Suzhou,Jiangsu 215123) |
| Abstract: | [Objective] The aim was to investigate the differences of PO activity and the mRNA expression during different developmental stages of Tenebrio molitor.[Method] Tenebrio molitor were chosen from each developmental stage(last instar larvae,pupae and adults) to be treated with three methods.The PO activity was measured and PPO expression was detected by semi-quantitative RT-PCR after 24h.[Result] PO activity increased with ontogeny,which was most vigorous in adults,next in pupae and then in last instar larvae... |
| Keywords: | Phenoloxidase Tenebrio molitor Semi-quantitative RT-PCR |
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