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| 杏ISSR反应体系的优化和指纹图谱的构建 | |
| 引用本文： | 刘威生,冯晨静,杨建民,刘冬成,张爱民,李绍华.杏ISSR反应体系的优化和指纹图谱的构建[J].果树学报,2005,22(6):626-629. |
| 作者姓名： | 刘威生 冯晨静 杨建民 刘冬成 张爱民 李绍华 |
| 作者单位： | 1. 中国农业大学农学与生物技术学院,北京,100094;辽宁省果树科学研究所,辽宁,熊岳,115009 2. 河北农业大学,河北,保定,071001 3. 中国科学院遗传与发育生物学研究所,北京,100101 4. 中国科学院植物研究所,北京,100093 |
| 摘 要： | 以杏品种清密沙为试材,用引物UBC825〔序列为(AC)8T〕研究了PCR反应体系的主要成分及退火温度对杏ISSR扩增结果的影响。结果表明:Primer、Mg2+、Taq酶浓度对扩增效果有明显影响,而模板DNA和dNTPs含量对扩增结果影响不大。优化的反应体系为:20μL的反应体系中含10ng模板DNA、0.1mmol/LdNTP、0.25μmol/LPrimer、2.5mmol/LMg2+,0.5UTaqPolymerase。适宜退火温度为50-52.1℃。用引物UBC825和UBC868〔序列为(GAA)5〕在优化的反应条件下建立了5个种12份杏材料的ISSR指纹图谱。 |
| 关 键 词： | 杏 ISSR(inter-simple sequence repeat) 反应体系 退火温度 优化 |
| 文章编号： | 1009-9980(2005)06-626-04 |
| 修稿时间： | 2005年5月20日 |
Optimization of ISSR reaction system and construction of cultivar fingerprint in apricot |
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| LIU Wei-sheng,FENG Chen-jing,YANG Jian-min,LIU Dong-cheng,ZHANG Ai-min,LI Shao-hua.Optimization of ISSR reaction system and construction of cultivar fingerprint in apricot[J].Journal of Fruit Science,2005,22(6):626-629. | |
| Authors: | LIU Wei-sheng FENG Chen-jing YANG Jian-min LIU Dong-cheng ZHANG Ai-min LI Shao-hua |
| Institution: | LIU Wei-sheng1,2,FENG Chen-jing3,YANG Jian-min3,LIU Dong-cheng4,ZHANG Ai-min4,LI Shaohua5* |
| Abstract: | The effects of the concentrations of PCR reaction components on inter-simple sequence repeat (ISSR) amplification results of apricot cv. Qingmisha were studied with the primer UBC825〔primer sequence: (AC)8T〕. The results showed that optimal concentrations of PCR-ISSR reaction system was 10 ng template DNA,2.5 mmol/L Mg2+,0.25 μmol/L Primer, 0.1 mmol/L dNTPs and 0.5 U Taq Polymerase per 20 μL reaction volume and the optimal annealing temperature was between 50℃ and 52.1℃. The ISSR fingerprinting profiles of 12 accessions covering five species of apricot were established with primer UBC825〔(AC)8T〕and primer UBC868〔(GAA)5〕. |
| Keywords: | Apricot ISSR(inter-simple sequence repeat) Reaction system Annealing temperature Optimization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |