| 一种适合香蕉根系总RNA提取的方法 |
| |
| 引用本文： | 王甲水,张建斌,贾彩虹,刘菊华,金志强.一种适合香蕉根系总RNA提取的方法[J].热带生物学报,2010,1(3):202-205,209. |
| |
| 作者姓名： | 王甲水 张建斌 贾彩虹 刘菊华 金志强 |
| |
| 作者单位： | 王甲水,WANG Jia-shui(中国热带农业科学院,热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海南,海口,571101;海南大学,农学院,海南,海口,570228);张建斌,贾彩虹,刘菊华,金志强,Zhang Jian-bing,Jia Cai-Hong,LIU Ju-hua,JIN Zhi-qiang(中国热带农业科学院,热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海南,海口,571101) |
| |
| 基金项目： | 公益性行业(农业)科研专项，现代农业产业技术体系建设专项资金资助 |
| |
| 摘 要： | 利用改良SDS法、改良CTAB法、SDS法和QIAGENR Neasy Plant Mini试剂盒提取法分别提取香蕉幼根和老根的总RNA,并对提取的总RNA的完整性、纯度等进行比较。结果表明：改良的SDS法提取的总RNA在清晰度、纯度、完整性和回收率上均高于其他3种方法所提取的。
|
| 关 键 词： | 香蕉根系 改良SDS法 植物总RNA 提取 |
A Protocol for Total RNA Extraction from the Roots of Banana |
| |
| WANG Jia-shui,Zhang Jian-bing,Jia Cai-Hong,LIU Ju-hua,JIN Zhi-qiang.A Protocol for Total RNA Extraction from the Roots of Banana[J].Journal of Tropical Biology,2010,1(3):202-205,209. |
| |
| Authors: | WANG Jia-shui Zhang Jian-bing Jia Cai-Hong LIU Ju-hua JIN Zhi-qiang |
| |
| Institution: | 1. Key Laboratory of Topical Crop Biotechnology,Ministry of Agriculture ,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Science,Haikou 571101,China;2. Department of Agriculture,Hainan University,Haikou 570228,China) |
| |
| Abstract: | The method of improved CTAB,SDS and QIAGEN RNeasy Plant Mini kit were used to isolate total RNA from juvenile and senescence banana roots respectively,and the quality,quantity and purity of total RNA were compared. The results showed that the quality,quantity and purity of total RNA isolated by improved SDS were superior to that by other methods. |
| |
| Keywords: | banana roots improved SDS method total RNA extraction |
| 本文献已被 维普 万方数据 等数据库收录！ |
|
