响应面法优化林蛙蛙皮中蛋白质的提取工艺

目的]利用响应面分析法优化林蛙皮中蛋白质的提取工艺。方法]通过单因素试验,确定响应面试验因素与中心水平。根据Box-Behnken试验设计原理采用4因素3水平的响应面法,依据回归分析,以蛋白质提取率为响应值作响应面,分析各因素的显著性和交互作用。结果]林蛙皮中蛋白质提取的最佳条件为:液料比30.35,提取时间3.28 h,提取温度11.13℃,pH为6.01,提取次数为2次。在最佳工艺条件下,蛋白质的实际提取率为6.11%。利用SDS-PAGE得到林蛙皮蛋白质分子量主要集中在43～20 kD。

Optimization of Extracting Conditions for Protein from Frog Skin(Rana Chensinensis) by Response Surface Methodology

Objective] The technology for extracting protein from frog skin was optimized by response surface methodology（RSM）.Method] The RSM experiment factors and levels were determined by single factor experiments.On the basis of Box-Behnken experiment design,a 4 factors and 3 levels’RSM was applied to get the RSM plot with the protein extraction rate as the response value after regression analysis.Then the significance and interaction of every factor were analyzed.Result]The optimum extracting conditions of protein were as follows： the ratio of water to material of 30.35,extracting time of 3.28 h,extracting temperature of 11.13 ℃,pH of 6.01,2 times.The actual extraction rate is 6.11% under the optimum conditions.Using SDS-PAGE,the main range of molecular weight of protein is 43-20 kD.