高产酒精的番茄酿酒酵母的选育研究

目的]筛选出产酒能力高、发酵速度快的番茄酿酒酵母突变株。方法]以从番茄表面分离筛选的产酒酵母HBF-2为出发菌株,分别利用紫外线(UV)和亚硝基胍(NTG)进行诱变,通过测定吸光度和致死率绘制出发菌株的生长曲线与致死曲线,根据诱变后菌落形态特征对菌种初筛,根据发酵酒的酒精度、残糖和总酸检测对菌种进行复筛,并对目的菌株进行遗传稳定性试验。结果]研究表明,出发菌株HBF-2生长周期为24 h,培养10 h后进入对数生长期;利用20 W紫外灯,在照射距离为40 cm条件下,照射时间为90 s,筛选出产酒能力比HBF-2提高了18.2%的突变株UV-8;UV-8连续传代5次,番茄发酵试验结果显示该菌株有良好的遗传稳定性。结论]研究可为番茄酒的酵母选育提供理论基础与参考。

Breeding of A High-yield Alcohol Strain Tomato Saccharomyce scerevisiae

Objective] To screen tomato Saccharomyces cerevisiae mutant with rapid fermentation speed and high ability of producing wine.Method] The ultraviolet and nitrosoguanidine was applied to HBF-2 which was isolated from tomato surface.The growth curve and death curve of the original strain were drawn by measuring the absorbance and death rate.The high-yield alcohol mutant strain was identified based on the colony morphology characteristics after mutagenic,and its contents of alcohol,residual sugar and total acid were measured to rescreen.And do the genetic stability experiments to the final bacteria.Result] The results indicated that the growth cycle of the original strain is 24 h and the log-phase occurs after being cultured for 10 h.The mutant UV-8 is screened out at the UV lamp is 20 W,the irradiation distance is 40 cm,and the irradiation time is 90 s.The ability of the mutant produced more 18.2% alcohol than the starting strain HBF-2.UV-8 was continuous passage cultured five times,tomato fermentation results illustrated that the strain has good genetic stability.Conclusion] The study can provide theoretical foundation and reference for yeast breeding of tomato wine.