响应面法优化超临界CO2萃取当归挥发油工艺及抑菌活性研究

目的]采用响应面法对超临界CO2萃取当归油的工艺进行优化,同时对当归挥发油的抑制活性进行研究。方法]在单因素试验基础上,采用Box-Behnken试验设计,考察萃取压力、萃取温度和萃取时间对挥发油提取率的影响,并采用纸片扩散法对提取的挥发油对的抑菌效果进行考察。结果]当归挥发油的最优提取工艺为:萃取温度44.4℃、萃取压力32.37 MPa、萃取时间1.48 h;在此条件下,萃取率可达2.083 3%。当归挥发油对部分常见的细菌和真菌具有较好的抑菌活性,MIC值在1.25～5.00 mg/ml范围内。结论]超临界CO2萃取当归油工艺稳定,提取率较高;当归油具有明显的抑菌活性,将为当归挥发油在抑菌制剂中的应用提供一定的理论依据。

Optimization of Supercritical Carbon Dioxide Extraction Conditions of Angelica sinensis Volatile Oil Using Response Surface Method and the Detection Research of Antimicrobial Activity

Objective] Response surface analysis method was applied to optimize the supercritical carbon dioxide extraction conditions of Angelica sinensis,the antimicrobial activity of Angelica sinensi volatile oil was studied.Method] Based on the single factor experiment,a three-factor-three-level experiment design was developed by Box-Benhken central composite design method.The influences of extraction temperature extraction time extraction pressure on the yeild of extraction were studied.The response surface method was employed to analyze the results of experiments.The disk diffusion method was used to detect the antimicrobial activity of Angelica sinensi volatile oil.Result] The results indicated that the optimum extraction conditions were as follows： extraction temperature 44.4 ℃,extraction time 1.48h,extraction pressure 32.37 Mpa,the extraction yield reached 2.0833%.The volatile oil of Angelica sinensis had antimicrobial activity of the tested strains,the MIC of the volatile oil was ranged 1.25 mg/ml from 5 mg/ml.Conclusion] The supercritical carbon dioxide extraction condition is a stability process to obtain a higher extraction yield.The Angelica sinensis volatile oil has antimicrobial activity which could provide certain theoretical basis for the application of volatile oil.