金黄色葡萄菌5型荚膜多糖偶联鞭毛蛋白疫苗对小鼠乳腺炎模型的保护作用 |
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引用本文: | 袁林,王贤卓,郭建军.金黄色葡萄菌5型荚膜多糖偶联鞭毛蛋白疫苗对小鼠乳腺炎模型的保护作用[J].安徽农业科学,2013(12):5344-5346. |
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作者姓名: | 袁林 王贤卓 郭建军 |
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作者单位: | 江西省科学院微生物研究所,江西南昌,330029;南昌大学生命科学与食品工程学院,江西南昌,330031 |
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基金项目: | 江西省科技支撑计划,江西省科学院项目 |
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摘 要: | 目的]金黄色葡萄菌5型荚膜多糖(CP5)分别与鼠伤寒沙门氏菌鞭毛蛋白Flagellin、牛血清白蛋白(BSA)进行了偶联,并在小鼠模型上比较了这2种偶联疫苗所引起的体液免疫和粘膜免疫反应。方法]以鼠伤寒沙门氏菌鞭毛蛋白为载体蛋白,与CP5进行化学偶联,并在小鼠乳腺部位免疫,然后通过检测小鼠乳汁中的抗体分析其对小鼠的免疫保护作用。结果]乳腺局部免疫后flagellin-CP5组在乳腺部位引起了sIgA为代表的粘膜免疫反应和IgG为主的体液免疫反应。CP5和鞭毛蛋白的偶联物能够诱导更高的体液免疫以及粘膜免疫反应。结论]鞭毛蛋白具有较强的免疫佐剂作用。
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关 键 词: | 5型荚膜多糖 鞭毛蛋白 粘膜免疫 佐剂 |
Protective Action of Coupling Staphylococcus aureus Capsular Polysaccharide 5 with Flagellin Vaccine on Mastitis Model of Mice |
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Institution: | YUAN Lin et al(Institute of Microbiology,Jiangxi Academy of Sciences,Nanchang,Jiangxi 330029) |
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Abstract: | Objective] Capsular polysaccharide 5 of Staphylococcus aureus(CP5) was coupled with Salmonella typhimurium flagellin and bovine serum albumin(BSA).And the humoral and mucosal immune responses elicited by these two coupled vaccines on mice model were compared.Method] S.typhimurium flagellin was used as carrier protein to make a chemical coupling with CP5 and immunize at mice breast.Then the antibody level in mice milk was detected to analyze its immune protective action on mice.Result]After breast immunization,mucosal immune responses with sIgA as representative and humoral immune responses with IgG as main were caused at breast part in flagellin-CP5 group.The conjugated compound of CP5 and flagellin could induce higher humoral and mucosal immune responses.Conclusion] Flagellin had strong adjuvant effects for capsular polysaccharide vaccine. |
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Keywords: | Capsular polysaccharide 5 Flagellin Mucosal immunity Adjuvant |
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