| 美洲大蠊Na+ -K+ -ATPase 作为筛选靶标的初步研究 |
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| 引用本文： | 蒋志胜,尚稚珍,王晓博,杨淑华.美洲大蠊Na+ -K+ -ATPase 作为筛选靶标的初步研究[J].农药学学报,2000,2(4):28-32. |
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| 作者姓名： | 蒋志胜 尚稚珍 王晓博 杨淑华 |
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| 作者单位： | 1.南开大学元素有机化学国家重点实验室, 元素有机化学研究所, 天津 300071 |
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| 摘 要： | 以美洲大蠊(Perip lanata am ericana) 雄性成虫的中枢神经系统为试材, 对Na+ -K+ -A TPase 作为农药筛选靶标进行了初步研究, 表明Na+ -K+ -A TPase 活力测定的最适反应条件是: 酶源蛋白浓度815 μg/m L , 温度30℃, pH7.4, 反应时间15min。同时以最佳反应系统初步研究了几种拟除虫菊酯(南开菊酯、氯氰菊酯、溴灭菊酯、氰戊菊酯、二氯苯醚菊酯、胺菊酯) 对Na+ -K+ -A TPase 离体活性的影响, 结果显示出不同程度的抑制作用。并对其用作农药筛选靶标的可能性进行了探讨。
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| 关 键 词： | Na+ -K+ -ATPase 筛选靶标 美洲大蠊 拟除虫菊酯 |
Preliminary Study on the Application of Na+ -K+ -ATPasein Adult Periplaneta americana as a Screening Target |
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| J iang Zh i-sheng,Shang Zh i-zhen,Wang Xiao-bo and Yang Shu-hua.Preliminary Study on the Application of Na+ -K+ -ATPasein Adult Periplaneta americana as a Screening Target[J].Chinese Journal of Pesticide Science,2000,2(4):28-32. |
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| Authors: | J iang Zh i-sheng Shang Zh i-zhen Wang Xiao-bo and Yang Shu-hua |
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| Institution: | 1.State Key Laboratory of Elemento Organic Chemistry , Institute of Elemento Organic Chemistry, Nankai University,Tianjin 300071 |
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| Abstract: | A screening model for pesticides was set up with Na + K + -ATPase from the central nervous system of adult Periplaneta americana. The optimum reaction condition of Na + K + ATPase was 5～10 μg/mL for the optimal protein oncentration,pH7.4,temperature 30 ℃ and time 15 min.Some pyrethroids were tested for their inhibitory activity on Na + K + -ATPase,the results showed that the screening model with Na+ K+ ATPase is sensitive,repetitive,labor-saving and sample-saving. |
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| Keywords: | Na+ K+ ATPase Screening target Periplaneta americana Pyrethroids |
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