利用cDNA微阵列分离橡胶胶乳生物合成相关基因的研究

天然橡胶是我国重要的热带经济作物，关系到国计民生的重要战略物资。本研究通过对橡胶叶与胶乳mRNA的提取，及其逆转录产物cDNA经Cy3、Cy5荧光染料标记后，进行芯片杂交，杂交数据经分析结果显示，在橡胶胶乳中表达上调达显著的基因有105个, 其中表达极显著的基因有44个, 包括elongation factor Tu(延伸因子基因), thioredoxin H-type 1（硫氧还蛋白H型1基因），4个zinc finger (C3HC4-type RING finger) family protein（锌指蛋白基因）等。在橡胶叶中表达显著的基因有151个, 其中表达极显著的有34个。研究结果对于发现橡胶胶乳表达相关基因有着重大的实践作用，对研究橡胶生物合成相关基因具有重要的参考作用，这将为研究橡胶生物合成机理奠定基础。

Screening the Genes Associated with Rubber Latex Biosynthesis Using cDNA Microarray

Abstract: The natural rubber is one of the more important industrial raw materials, also it is important and strategic supplies which relates to the national security and economic development. In this study, mRNAs were isolated form the tissues of rubber latex and rubber leaves, and their cDNA were hybridized with microarray, and the results indicted that 105 genes were up-regulated in rubber latex, including elongation factor Tu, sterol 24-C-methyltransferase，thioredoxin H-type 1， zinc finger (C3HC4-type RING finger) family protein etc. and 151 genes were down- regulated in rubber leaves. The results have the significant practice on discovering rubber latex expression related genes and rubber biosynthesis-related genes, and which make a good foundation to study the mechanism of rubber biosynthesis.