猪囊尾蚴“四性细胞系”代射产物（可溶性抗原）的免疫原性

应用仔猪20头,以猪囊尾蚴“四性细胞系”的可溶性抗原作为免疫原,对经过第1次用不同细胞剂量疫苗免疫的猪,再次用与细胞数量相适应的细胞代射产物的不同剂量疫苗,对其中的11头进行第2次免疫以第2次还是注射细胞疫苗的3头、只注射1次细胞疫苗的2头,和空白对照猪2头作对照组,以观察代射产物抗原的免疫原性。于第1次细胞疫苗免疫后的68d,最长94d用细胞加代谢产物疫苗进行了这次免疫,14d后攻虫,每头使用人有钩绦虫卵,6000枚,3个月后屠宰检查虫体。第1次用10万细胞疫苗免疫,第2次用1千万细胞加代谢产物疫苗免疫该组动物中的2头,平衡有5个囊尾蚴,但这2头猪均发生了囊尾蚴钙化;另一组第1次用100万细胞疫苗免疫,第2次用2千万细胞加代谢产物疫苗免疫该组全部3头动物,平均有3.6个囊尾蚴,这3头猪也都有囊尾蚴钙化。2个实验组出现免疫猪的100%的钙化现象,是免疫治疗作用,是猪囊尾蚴四性细胞系代谢产物的特殊免疫作用。

Immunogenicity of the Metabolite of "Four Features Cell Line"of Cysticercus Cellulosae

The research applied 20 subject animals(pigs), and took solubility antigen of "four features cell line"of Cysticercus cellulosae as immune antigen to immunize the pigs by the vaccine with different dosage at the first time,and to immunize the 11 of the 18 pigs by the vaccine with different dosage of cell and its corresponding metabolite at the second time.Using 3 which were injected with cell vaccine twice,2 injected only once and 2 of control pigs,we make control experiment to observe the immunogenicity of metabolite antigen. We immunized the pigs the second time with cell adding its metabolite after 68,94 days of immunization with the cell vaccine the first time.Each of the pigs was attacked larvae by 6 000 ova.After 3 months,we killed and examined the Cysticercuses and found that at the first time,we immunized 2 pigs with 100 000 cell vaccine,the second time with 10 000 000 cells adding their metabolites,and found 5 Cysticercuses at average. But the Cysticercus cellulosae infecting the 2 pigs had been calcified. Another 3 pigs were immunized with 1 000 000 cell vaccine at the first time,and the second time with 20 000 000 cells adding their metabolites. We found 3.6 Cysticercuses at average,and these Cysticercus cellulosae were also calcified.The calcification of Cysticercus cellolusae is a special immune effect of the metabolite of "four features cell line"of Cysticercus cellulosae.