|
|||||
|
|
| 表达PRRSV E基因重组质粒在小鼠诱导的体液免疫应答 | |
| 引用本文： | 高云,杨汉春,刘平黄,陈声,郭鑫,韩军,黄芳芳. 表达PRRSV E基因重组质粒在小鼠诱导的体液免疫应答[J]. 中国兽医杂志, 2004, 40(11): 6-8 |
| 作者姓名： | 高云 杨汉春 刘平黄 陈声 郭鑫 韩军 黄芳芳 |
| 作者单位： | 中国农业大学农业部预防兽医学重点开放实验室,北京,海淀,100094 |
| 基金项目： | 教育部霍英东青年教师基金资助，国家十五攻关子课题 ( 2 0 0 2 BA5 14 A-16-0 5 ) |
| 摘 要： | 利用 PCR技术对猪繁殖与呼吸综合征病毒 (PRRSV) BJ- 4株的 E基因进行修饰和改造 ,在 E基因上游加入 Kozak序列 ,扩增并克隆 E基因。将 E基因 c DNA亚克隆至真核表达载体 pc DNA3.1( )中 ,构建了真核重组表达质粒 pc DNA- E。用pc DNA- E免疫小鼠 ,经免疫荧光抗体试验检测结果表明 ,重组质粒 pc DNA- E经 3次免疫后 ,所有小鼠血清抗体均为阳性 ,说明pc DNA- E在小鼠体内可诱导特异性的体液免疫应答反应。 |
| 关 键 词： | 猪繁殖与呼吸综合征病毒 E基因 重组质粒 体液免疫应答 |
| 文章编号： | 0529-6005(2004)11-0006-03 |
| 修稿时间： | 2003-12-09 |
Humoral immune response evicited by the recombinant plasmid expressing E gene of PRRSV in mice |
|
| GAO Yun,YANG Han-chun,LIU Ping-huang,CHEN Sheng,GUO Xin,HAN Jun,HUANG Fang-fang. Humoral immune response evicited by the recombinant plasmid expressing E gene of PRRSV in mice[J]. Chinese Journal of Veterinary Medicine, 2004, 40(11): 6-8 | |
| Authors: | GAO Yun YANG Han-chun LIU Ping-huang CHEN Sheng GUO Xin HAN Jun HUANG Fang-fang |
| Abstract: | By PCR, the ORF5 (E gene) of PRRS virus BJ-4 strain was modified and amplified, and sub-cloned into expression vector pcDNA3.1( ). The recombinant expression plasmid pcDNA-E was inoculated into mice. The antibodies specific for E protein in serum were confirmed by IFA. The antibody could be detected in all the mice after the third inoculation.The results showed that the recombinant plasmid pcDNA-E induced specific humoral immune responses in mice. |
| Keywords: | PRRS virus E gene recombinant plasmid humoral immune response |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |