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| 影响不结球白菜真空渗入转基因频率的几个因素 | |
| 引用本文： | 曹传增,刘凡,赵泓,蔡民华,徐恒戬.影响不结球白菜真空渗入转基因频率的几个因素[J].华北农学报,2003,18(4):35-38. |
| 作者姓名： | 曹传增 刘凡 赵泓 蔡民华 徐恒戬 |
| 作者单位： | 1. 首都师范大学,生物系,北京,100037 2. 北京蔬菜研究中心,北京,100089 3. 山东农业大学,园艺学院,山东,泰安,271018 |
| 摘 要： | 采用不同发育阶段的植物材料及不同菌株，将马铃薯胰蛋白酶抑制剂基因(PinⅡ)和蜘蛛杀虫肽(SIP)基因双价转入49菜心，以除草剂抗性株出现频率，以及目的基因的PCR鉴定结果为依据，对影响不结球白菜-49菜心真空渗入转化率的一些因素进行了探索。结果表明植株生长阶段和真空渗入处理时间、所用菌株类型均对转化率有影响，其中以开花初期的植株，利用胭脂碱型农杆菌介导，真空渗入5min的处理方法转化效率最高。 |
| 关 键 词： | 不结球白菜 真空渗入法 转基因 生产频率 影响因素 |
| 文章编号： | 1000-7091(2003)04-0035-04 |
| 修稿时间： | 2003年3月10日 |
Several Factors Affecting the Vacuum Infiltration Gene Transformation in Non-heading Chinese Cabbage |
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| CAO Chuan-zeng.Several Factors Affecting the Vacuum Infiltration Gene Transformation in Non-heading Chinese Cabbage[J].Acta Agriculturae Boreali-Sinica,2003,18(4):35-38. | |
| Authors: | CAO Chuan-zeng |
| Institution: | CAO Chuan-zeng~ |
| Abstract: | Several factors affecting the vacuum infiltration transformation efficiency in Chinese cabbage (Brassica parachinensis Bailey) were studied.The developmental status of seedlings,vacuum infiltration time,type of Agrobacterium tumefaciens used,all influenced the transformation frequency.Via selection by herbicide Basta in the progeny and identification of the occurrence of the target gene by PCR, the transformation frequency was compared in different treatments.It was concluded that using the nopaline type of Agrobacterium,5 min vacuum infiltration,and using the plants with just several blooming flowers would give the best results. |
| Keywords: | Vacuum infiltration transformation Insect-resistant genes Non-heading Chinese cabbage |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |