益气活血中药对糖尿病动脉粥样硬化家兔血管平滑肌细胞凋亡及Bcl-2表达的影响

目的探讨益气活血中药对糖尿病动脉粥样硬化家兔血管平滑肌细胞凋亡及凋亡相关基因Bcl-2表达的影响。方法选用新西兰家兔,利用静脉注射胰岛β细胞毒剂四氧嘧啶,继以高糖高脂饲料复制家兔糖尿病动脉粥样硬化模型,随机分为模型组、小剂量中药治疗组、大剂量中药治疗组和正常对照组,实验12周后,处死家兔,观察主动脉内膜的病理形态学改变,采用流式细胞术检测凋亡率以及凋亡相关基因Bcl-2表达。结果模型组与正常对照组比较,动脉内膜出现典型的斑块,动脉管腔极度狭窄,平滑肌细胞凋亡率明显增高(13.89%±1.53%比2.36%±0.76%,P<0.01),Bcl-2表达降低(22.04±1.38比30.48±0.68,P<0.01)。中药治疗组与模型组比较,主动脉斑块缩小,动脉管腔狭窄减轻,平滑肌细胞凋亡率明显降低(P<0.01),Bcl-2表达上调(P<0.05),且大剂量中药治疗组优于小剂量中药治疗组。结论益气活血中药拮抗糖尿病动脉粥样硬化形成的机制可能与上调Bcl-2蛋白的表达,抑制血管平滑肌细胞凋亡有关。

The Effect of Nourishing Qi and Promoting Blood Herbs on the Apoptosis of Vascular Smooth Muscle Cell and Expression of Bcl-2 in Rabbit Model with Atherosclerosis and Diabetes

Aim To approach the effect of nourishing qi and promoting blood herbs on the apoptosis of vascular smooth muscle cell(VSMC) and expression of Bcl-2 in rabbit model with arteriosclerosis and diabetes.Methods By injecting tetraoxypyrimidine intravenous and breeding high glucose and grease animal feeds,the rabbit animal with arteriosclerosis and diabetes can be obtained.The models were divided into three groups randomly:model group,large dose and low dose treatment group,controlled with the normal group.After 12 weeks,all the animals were killed.The pathomorphology of intima of aorta were observed,and the apoptotic rate and expression of Bcl-2 were detected by flow cytometry.Results In the model group the apoptotic rate of smooth muscle cell was higher than that in the normal group(P<0.01),but the expression of Bcl-2 was lower(P<0.01).The visible atheromatous plaque which caused the serious stenosis were observed in model group.In the treatment group the apoptotic rate of smooth muscle cell was lower than that in the model group(P<0.01),but the expression of Bcl-2 was higher(P<0.01),the atheromatous plaque were decreased,and the stenosis were reduced,which were more obvious in the large dose treatment group than in the low dose treatment group.Conclusion The nourishing qi and promoting blood herbs can influence the apoptosis of VSMC and expression of Bcl-2 in rabbit model with arteriosclerosis and diabetes.The herbs have the better treatment on the diabetic arteriosclerosis obliterans.